Bert Poolman Department of Biochemistry University of Groningen Biography Publications Institution JoVE Articles Bert Poolman has not added a biography. If you are Bert Poolman and would like to personalize this page please email our Author Liaison for assistance. Publications Macromolecular Crowding, Phase Separation, and Homeostasis in the Orchestration of Bacterial Cellular Functions Chemical Reviews. Feb, 2024 | Pubmed ID: 38331392 Single-protein Diffusion in the Periplasm of Escherichia Coli Journal of Molecular Biology. Feb, 2024 | Pubmed ID: 38143021 Simulation-based Reconstructed Diffusion Unveils the Effect of Aging on Protein Diffusion in Escherichia Coli PLoS Computational Biology. Sep, 2023 | Pubmed ID: 37695774 Physicochemical Homeostasis in Bacteria FEMS Microbiology Reviews. Jul, 2023 | Pubmed ID: 37336577 Minimal Out-of-Equilibrium Metabolism for Synthetic Cells: A Membrane Perspective ACS Synthetic Biology. Apr, 2023 | Pubmed ID: 37027340 Super-resolving Microscopy Reveals the Localizations and Movement Dynamics of Stressosome Proteins in Listeria Monocytogenes Communications Biology. Jan, 2023 | Pubmed ID: 36641529 Determining Small-molecule Permeation Through Lipid Membranes Nature Protocols. Nov, 2022 | Pubmed ID: 36002767 Protein Diffusion in Cytoplasm Scales with the Mass of the Complexes and is Location Dependent Science Advances. Aug, 2022 | Pubmed ID: 35960807 Natamycin Sequesters Ergosterol and Interferes with Substrate Transport by the Lysine Transporter Lyp1 from Yeast Biochimica Et Biophysica Acta. Biomembranes. Nov, 2022 | Pubmed ID: 35914570 ATP Recycling Fuels Sustainable Glycerol 3-Phosphate Formation in Synthetic Cells Fed by Dynamic Dialysis ACS Synthetic Biology. Jul, 2022 | Pubmed ID: 35377147 Membrane Thickness, Lipid Phase and Sterol Type Are Determining Factors in the Permeability of Membranes to Small Solutes Nature Communications. Mar, 2022 | Pubmed ID: 35338137 Minimal Pathway for the Regeneration of Redox Cofactors JACS Au. Dec, 2021 | Pubmed ID: 34984417 Fluorescence-based Sensing of the Bioenergetic and Physicochemical Status of the Cell Current Topics in Membranes. 2021 | Pubmed ID: 34862023 Heterodimer Formation of the Homodimeric ABC Transporter OpuA International Journal of Molecular Sciences. May, 2021 | Pubmed ID: 34072847 Stability of Ligand-induced Protein Conformation Influences Affinity in Maltose-binding Protein Journal of Molecular Biology. Jul, 2021 | Pubmed ID: 33957147 Chemogenetic Tags with Probe Exchange for Live-Cell Fluorescence Microscopy ACS Chemical Biology. May, 2021 | Pubmed ID: 33913682 Glass-like Characteristics of Intracellular Motion in Human Cells Biophysical Journal. Jun, 2021 | Pubmed ID: 33887228 Structural and Biophysical Characterization of the Tandem Substrate-binding Domains of the ABC Importer GlnPQ Open Biology. Apr, 2021 | Pubmed ID: 33823661 In Silico Method for Selecting Residue Pairs for Single-molecule Microscopy and Spectroscopy Scientific Reports. Mar, 2021 | Pubmed ID: 33707507 Measurement of Protein Mobility in Reveals a Unique Tolerance to Osmotic Stress and Temperature Dependence of Diffusion Frontiers in Microbiology. 2021 | Pubmed ID: 33679676 Energy Coupling of Membrane Transport and Efficiency of Sucrose Dissimilation in Yeast Metabolic Engineering. May, 2021 | Pubmed ID: 33279674 Enzymology of the Pathway for ATP Production by Arginine Breakdown The FEBS Journal. Jan, 2021 | Pubmed ID: 32306469 Growth Inhibition by Amino Acids in Microorganisms. Dec, 2020 | Pubmed ID: 33375077 Gating by Ionic Strength and Safety Check by Cyclic-di-AMP in the ABC Transporter OpuA Science Advances. Nov, 2020 | Pubmed ID: 33208376 Structural and Functional Diversity Calls for a New Classification of ABC Transporters FEBS Letters. 12, 2020 | Pubmed ID: 32978974 Kinetic Modelling of Transport Inhibition by Substrates in ABC Importers Journal of Molecular Biology. Sep, 2020 | Pubmed ID: 32800784 Stereoselective Protection-Free Modification of 3-Keto-saccharides Organic Letters. Jul, 2020 | Pubmed ID: 32635733 Membrane Lipid Requirements of the Lysine Transporter Lyp1 from Saccharomyces Cerevisiae Journal of Molecular Biology. Jun, 2020 | Pubmed ID: 32413406 Periprotein Lipidomes of Provide a Flexible Environment for Conformational Changes of Membrane Proteins ELife. Apr, 2020 | Pubmed ID: 32301705 Extracellular Loops Matter - Subcellular Location and Function of the Lysine Transporter Lyp1 from Saccharomyces cerevisiae The FEBS Journal. Oct, 2020 | Pubmed ID: 32096906 Physiochemical Modeling of Vesicle Dynamics Upon Osmotic Upshift Biophysical Journal. Jan, 2020 | Pubmed ID: 31948692 Weak Acid Permeation in Synthetic Lipid Vesicles and Across the Yeast Plasma Membrane Biophysical Journal. Jan, 2020 | Pubmed ID: 31843263 A Trifunctional Linker for Palmitoylation and Peptide and Protein Localization in Biological Membranes Chembiochem : a European Journal of Chemical Biology. May, 2020 | Pubmed ID: 31814256 Flexible and Extended Linker Domains Support Efficient Targeting of Heh2 to the Inner Nuclear Membrane Structure (London, England : 1993). Feb, 2020 | Pubmed ID: 31806352 Physicochemical Considerations for Bottom-up Synthetic Biology Emerging Topics in Life Sciences. Nov, 2019 | Pubmed ID: 33523159 Regulation of Amino Acid Transport in Saccharomyces Cerevisiae Microbiology and Molecular Biology Reviews : MMBR. Nov, 2019 | Pubmed ID: 31619504 A Synthetic Metabolic Network for Physicochemical Homeostasis Nature Communications. Sep, 2019 | Pubmed ID: 31534136 Cell Fuelling and Metabolic Energy Conservation in Synthetic Cells Chembiochem : a European Journal of Chemical Biology. Oct, 2019 | Pubmed ID: 31381223 Conformational and Dynamic Plasticity in Substrate-binding Proteins Underlies Selective Transport in ABC Importers ELife. Mar, 2019 | Pubmed ID: 30900991 Decreased Effective Macromolecular Crowding in Escherichia Coli Adapted to Hyperosmotic Stress Journal of Bacteriology. May, 2019 | Pubmed ID: 30833357 Adaption to Glucose Limitation is Modulated by the Pleotropic Regulator CcpA, Independent of Selection Pressure Strength BMC Evolutionary Biology. Jan, 2019 | Pubmed ID: 30630406 Coupling Efficiency of Secondary Active Transporters Current Opinion in Biotechnology. Aug, 2019 | Pubmed ID: 30502621 How Important Is Protein Diffusion in Prokaryotes? Frontiers in Molecular Biosciences. 2018 | Pubmed ID: 30483513 (Membrane) Protein Production in Context Trends in Biochemical Sciences. Nov, 2018 | Pubmed ID: 30220516 Method for Immobilization of Living and Synthetic Cells for High-resolution Imaging and Single-particle Tracking Scientific Reports. Sep, 2018 | Pubmed ID: 30213985 Influence of Fluorescent Protein Maturation on FRET Measurements in Living Cells ACS Sensors. Sep, 2018 | Pubmed ID: 30168711 Niosomes, an Alternative for Liposomal Delivery PloS One. 2018 | Pubmed ID: 29649223 Protein Linkers Provide Limits on the Domain Interactions in the ABC Importer GlnPQ and Determine the Rate of Transport Journal of Molecular Biology. Apr, 2018 | Pubmed ID: 29486154 Energy Coupling Efficiency in the Type I ABC Transporter GlnPQ Journal of Molecular Biology. Mar, 2018 | Pubmed ID: 29432725 Steric Exclusion and Protein Conformation Determine the Localization of Plasma Membrane Transporters Nature Communications. Feb, 2018 | Pubmed ID: 29402931 Combined Engineering of Disaccharide Transport and Phosphorolysis for Enhanced ATP Yield from Sucrose Fermentation in Saccharomyces Cerevisiae Metabolic Engineering. Jan, 2018 | Pubmed ID: 29196124 Real-Time Conformational Changes and Controlled Orientation of Native Proteins Inside a Protein Nanoreactor Journal of the American Chemical Society. Dec, 2017 | Pubmed ID: 29206456 Ribosome Surface Properties May Impose Limits on the Nature of the Cytoplasmic Proteome ELife. Nov, 2017 | Pubmed ID: 29154755 Proton-solute Coupling Mechanism of the Maltose Transporter from Saccharomyces Cerevisiae Scientific Reports. Oct, 2017 | Pubmed ID: 29084970 A General Mechanism of Ribosome Dimerization Revealed by Single-particle Cryo-electron Microscopy Nature Communications. Sep, 2017 | Pubmed ID: 28959045 Ionic Strength Sensing in Living Cells ACS Chemical Biology. Oct, 2017 | Pubmed ID: 28853549 Design and Properties of Genetically Encoded Probes for Sensing Macromolecular Crowding Biophysical Journal. May, 2017 | Pubmed ID: 28494963 Microorganisms Maintain Crowding Homeostasis Nature Reviews. Microbiology. May, 2017 | Pubmed ID: 28344349 On the Spatial Organization of MRNA, Plasmids, and Ribosomes in a Bacterial Host Overexpressing Membrane Proteins PLoS Genetics. Dec, 2016 | Pubmed ID: 27977669 An Updated Structural Classification of Substrate-binding Proteins FEBS Letters. Dec, 2016 | Pubmed ID: 27714801 On the Mobility, Membrane Location and Functionality of Mechanosensitive Channels in Escherichia Coli Scientific Reports. Sep, 2016 | Pubmed ID: 27596282 Asymmetry in Inward- and Outward-affinity Constant of Transport Explain Unidirectional Lysine Flux in Saccharomyces Cerevisiae Scientific Reports. Aug, 2016 | Pubmed ID: 27550794 A Plasma Membrane Association Module in Yeast Amino Acid Transporters The Journal of Biological Chemistry. Jul, 2016 | Pubmed ID: 27226538 Relative Rates of Amino Acid Import Via the ABC Transporter GlnPQ Determine the Growth Performance of Lactococcus Lactis Journal of Bacteriology. Feb, 2016 | Pubmed ID: 26553850 Watching Conformational Dynamics of ABC Transporters with Single-molecule Tools Biochemical Society Transactions. Oct, 2015 | Pubmed ID: 26517920 Active Nuclear Import of Membrane Proteins Revisited Cells. Oct, 2015 | Pubmed ID: 26473931 Emergence of Life: Physical Chemistry Changes the Paradigm Biology Direct. Jun, 2015 | Pubmed ID: 26059688 Engineering Escherichia Coli for Functional Expression of Membrane Proteins Methods in Enzymology. 2015 | Pubmed ID: 25857775 Protein Costs Do Not Explain Evolution of Metabolic Strategies and Regulation of Ribosomal Content: Does Protein Investment Explain an Anaerobic Bacterial Crabtree Effect? Molecular Microbiology. Jul, 2015 | Pubmed ID: 25828364 In Memoriam: Wilhelmus Nicolaas Konings (1937-2014) Extremophiles : Life Under Extreme Conditions. Mar, 2015 | Pubmed ID: 25666832 Single Liposome Analysis of Peptide Translocation by the ABC Transporter TAPL Proceedings of the National Academy of Sciences of the United States of America. Feb, 2015 | Pubmed ID: 25646430 A Sensor for Quantification of Macromolecular Crowding in Living Cells Nature Methods. Mar, 2015 | Pubmed ID: 25643150 Size-dependent Leak of Soluble and Membrane Proteins Through the Yeast Nuclear Pore Complex Molecular Biology of the Cell. Apr, 2015 | Pubmed ID: 25631821 Conformational Dynamics in Substrate-binding Domains Influences Transport in the ABC Importer GlnPQ Nature Structural & Molecular Biology. Jan, 2015 | Pubmed ID: 25486304 Intrinsically Disordered Linker and Plasma Membrane-binding Motif Sort Ist2 and Ssy1 to Junctions Traffic (Copenhagen, Denmark). Feb, 2015 | Pubmed ID: 25409870 Disaccharides Impact the Lateral Organization of Lipid Membranes Journal of the American Chemical Society. Nov, 2014 | Pubmed ID: 25316578 Impact of Osmotic Stress on Protein Diffusion in Lactococcus Lactis Molecular Microbiology. Nov, 2014 | Pubmed ID: 25244659 Systematic Identification of TRNAome and Its Dynamics in Lactococcus Lactis Molecular Microbiology. Sep, 2014 | Pubmed ID: 25040919 Susceptibility to COPD: Differential Proteomic Profiling After Acute Smoking PloS One. 2014 | Pubmed ID: 25036363 Bacillus Subtilis Spore Protein SpoVAC Functions As a Mechanosensitive Channel Molecular Microbiology. May, 2014 | Pubmed ID: 24666282 Regulation of Acetate Kinase Isozymes and Its Importance for Mixed-acid Fermentation in Lactococcus Lactis Journal of Bacteriology. Apr, 2014 | Pubmed ID: 24464460 Lactococcus Lactis YfiA is Necessary and Sufficient for Ribosome Dimerization Molecular Microbiology. Jan, 2014 | Pubmed ID: 24279750 Evolved Escherichia Coli Strains for Amplified, Functional Expression of Membrane Proteins Journal of Molecular Biology. Jan, 2014 | Pubmed ID: 24041572 Functional Diversity of Tandem Substrate-binding Domains in ABC Transporters from Pathogenic Bacteria Structure (London, England : 1993). Oct, 2013 | Pubmed ID: 23994008 Physicochemical Factors Controlling the Activity and Energy Coupling of an Ionic Strength-gated ATP-binding Cassette (ABC) Transporter The Journal of Biological Chemistry. Oct, 2013 | Pubmed ID: 23979139 Dual Action of BPC194: a Membrane Active Peptide Killing Bacterial Cells PloS One. 2013 | Pubmed ID: 23620763 Quantitative Analysis of Membrane Protein Transport Across the Nuclear Pore Complex Traffic (Copenhagen, Denmark). May, 2013 | Pubmed ID: 23357007 On the Role of Individual Subunits in MscL Gating: "all for One, One for All?" FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology. Mar, 2013 | Pubmed ID: 23193173 Impact of Lactobacillus Plantarum Sortase on Target Protein Sorting, Gastrointestinal Persistence, and Host Immune Response Modulation Journal of Bacteriology. Feb, 2013 | Pubmed ID: 23175652 Structure and Mode of Peptide Binding of Pheromone Receptor PrgZ The Journal of Biological Chemistry. Oct, 2012 | Pubmed ID: 22948145 Structural Divergence of Paralogous S Components from ECF-type ABC Transporters Proceedings of the National Academy of Sciences of the United States of America. Aug, 2012 | Pubmed ID: 22891302 Effect of ITRAQ Labeling on the Relative Abundance of Peptide Fragment Ions Produced by MALDI-MS/MS Journal of Proteome Research. Aug, 2012 | Pubmed ID: 22770492 Functional Characterization of Amphipathic α-helix in the Osmoregulatory ABC Transporter OpuA Biochemistry. Jun, 2012 | Pubmed ID: 22656643 The Transport of Integral Membrane Proteins Across the Nuclear Pore Complex Nucleus (Austin, Tex.). Jul, 2012 | Pubmed ID: 22614756 Evaluation of Pulsed-FRAP and Conventional-FRAP for Determination of Protein Mobility in Prokaryotic Cells PloS One. 2011 | Pubmed ID: 21980523 The Response of Lactococcus Lactis to Membrane Protein Production PloS One. 2011 | Pubmed ID: 21904605 Cystathionine β-synthase (CBS) Domains 1 and 2 Fulfill Different Roles in Ionic Strength Sensing of the ATP-binding Cassette (ABC) Transporter OpuA The Journal of Biological Chemistry. Oct, 2011 | Pubmed ID: 21878634 Efficient Overproduction of Membrane Proteins in Lactococcus Lactis Requires the Cell Envelope Stress Sensor/regulator Couple CesSR PloS One. 2011 | Pubmed ID: 21818275 The Structural Basis of Modularity in ECF-type ABC Transporters Nature Structural & Molecular Biology. Jun, 2011 | Pubmed ID: 21706007 Design and Synthesis of ATP-based Nucleotide Analogues and Profiling of Nucleotide-binding Proteins Bioconjugate Chemistry. Jul, 2011 | Pubmed ID: 21692528 Importance of a Hydrophobic Pocket for Peptide Binding in Lactococcal OppA Journal of Bacteriology. Aug, 2011 | Pubmed ID: 21665971 Long Unfolded Linkers Facilitate Membrane Protein Import Through the Nuclear Pore Complex Science (New York, N.Y.). Jul, 2011 | Pubmed ID: 21659568 Structural Basis for the Enhanced Activity of Cyclic Antimicrobial Peptides: the Case of BPC194 Biochimica Et Biophysica Acta. Sep, 2011 | Pubmed ID: 21586269 The Molecular Basis for Antimicrobial Activity of Pore-forming Cyclic Peptides Biophysical Journal. May, 2011 | Pubmed ID: 21575576 De Novo Design of Supercharged, Unfolded Protein Polymers, and Their Assembly into Supramolecular Aggregates Macromolecular Rapid Communications. Jan, 2011 | Pubmed ID: 21433138 Quaternary Structure of SecA in Solution and Bound to SecYEG Probed at the Single Molecule Level Structure (London, England : 1993). Mar, 2011 | Pubmed ID: 21397193 Lipid Packing Drives the Segregation of Transmembrane Helices into Disordered Lipid Domains in Model Membranes Proceedings of the National Academy of Sciences of the United States of America. Jan, 2011 | Pubmed ID: 21205902 Macromolecule Diffusion and Confinement in Prokaryotic Cells Current Opinion in Biotechnology. Feb, 2011 | Pubmed ID: 20952181 Apex Peptide Elution Chain Selection: a New Strategy for Selecting Precursors in 2D-LC-MALDI-TOF/TOF Experiments on Complex Biological Samples Journal of Proteome Research. Nov, 2010 | Pubmed ID: 20836568 Lateral Diffusion of Membrane Proteins: Consequences of Hydrophobic Mismatch and Lipid Composition Biophysical Journal. Sep, 2010 | Pubmed ID: 20816060 Influence of Hydrophobic Mismatch and Amino Acid Composition on the Lateral Diffusion of Transmembrane Peptides Biophysical Journal. Sep, 2010 | Pubmed ID: 20816056 Genome Sequences of Lactococcus Lactis MG1363 (revised) and NZ9000 and Comparative Physiological Studies Journal of Bacteriology. Nov, 2010 | Pubmed ID: 20639323 Evolved Lactococcus Lactis Strains for Enhanced Expression of Recombinant Membrane Proteins Journal of Molecular Biology. Aug, 2010 | Pubmed ID: 20542040 Ligand Binding and Crystal Structures of the Substrate-binding Domain of the ABC Transporter OpuA PloS One. Apr, 2010 | Pubmed ID: 20454456 Amino Acid Accumulation Limits the Overexpression of Proteins in Lactococcus Lactis PloS One. Apr, 2010 | Pubmed ID: 20436673 Purification and Functional Reconstitution of the Bacterial Protein Translocation Pore, the SecYEG Complex Methods in Molecular Biology (Clifton, N.J.). 2010 | Pubmed ID: 20419408 A Structural Classification of Substrate-binding Proteins FEBS Letters. Jun, 2010 | Pubmed ID: 20412802 A Karyopherin Acts in Localized Protein Synthesis Cell Cycle (Georgetown, Tex.). Apr, 2010 | Pubmed ID: 20305392 Production of Membrane Proteins in Escherichia Coli and Lactococcus Lactis Methods in Molecular Biology (Clifton, N.J.). 2010 | Pubmed ID: 20099137 Proteomics of Saccharomyces Cerevisiae Organelles Molecular & Cellular Proteomics : MCP. Mar, 2010 | Pubmed ID: 19955081 Lateral Diffusion of Membrane Proteins Journal of the American Chemical Society. Sep, 2009 | Pubmed ID: 19673517 The Role of Biomacromolecular Crowding, Ionic Strength, and Physicochemical Gradients in the Complexities of Life's Emergence Microbiology and Molecular Biology Reviews : MMBR. Jun, 2009 | Pubmed ID: 19487732 Selenomethionine Incorporation in Proteins Expressed in Lactococcus Lactis Protein Science : a Publication of the Protein Society. May, 2009 | Pubmed ID: 19388077 A Method for Site-specific Labeling of Multiple Protein Thiols Protein Science : a Publication of the Protein Society. May, 2009 | Pubmed ID: 19388048 Engineering of Ion Sensing by the Cystathionine Beta-synthase Module of the ABC Transporter OpuA The Journal of Biological Chemistry. May, 2009 | Pubmed ID: 19329426 The Structural Basis for Peptide Selection by the Transport Receptor OppA The EMBO Journal. May, 2009 | Pubmed ID: 19300437 Nuclear Transport Factor Directs Localization of Protein Synthesis During Mitosis Nature Cell Biology. Mar, 2009 | Pubmed ID: 19198597 The Substrate-binding Protein Imposes Directionality on an Electrochemical Sodium Gradient-driven TRAP Transporter Proceedings of the National Academy of Sciences of the United States of America. Feb, 2009 | Pubmed ID: 19179287 Matching the Proteome to the Genome: the Microbody of Penicillin-producing Penicillium Chrysogenum Cells Functional & Integrative Genomics. May, 2009 | Pubmed ID: 19156454 Domain Complementation Studies Reveal Residues Critical for the Activity of the Mannitol Permease from Escherichia Coli Biochimica Et Biophysica Acta. Feb, 2009 | Pubmed ID: 19013424 The Yeast Vacuolar Membrane Proteome Molecular & Cellular Proteomics : MCP. Feb, 2009 | Pubmed ID: 19001347 Proteomic Analysis of Secreted Membrane Vesicles of Archaeal Sulfolobus Species Reveals the Presence of Endosome Sorting Complex Components Extremophiles : Life Under Extreme Conditions. Jan, 2009 | Pubmed ID: 18972064 On the Mechanism of Pore Formation by Melittin The Journal of Biological Chemistry. Dec, 2008 | Pubmed ID: 18819911 Transport and Sorting of the Solanum Tuberosum Sucrose Transporter SUT1 is Affected by Posttranslational Modification The Plant Cell. Sep, 2008 | Pubmed ID: 18790827 Dual-color Fluorescence-burst Analysis to Study Pore Formation and Protein-protein Interactions Methods (San Diego, Calif.). Oct, 2008 | Pubmed ID: 18667165 9th International Symposium on Lactic Acid Bacteria Applied and Environmental Microbiology. Aug, 2008 | Pubmed ID: 18586976 Quality Control of Overexpressed Membrane Proteins Proceedings of the National Academy of Sciences of the United States of America. Apr, 2008 | Pubmed ID: 18391190 Membrane Reconstitution of ABC Transporters and Assays of Translocator Function Nature Protocols. 2008 | Pubmed ID: 18274528 Probing Receptor-translocator Interactions in the Oligopeptide ABC Transporter by Fluorescence Correlation Spectroscopy Biophysical Journal. May, 2008 | Pubmed ID: 18212011 High-throughput Cloning and Expression in Recalcitrant Bacteria Nature Methods. Sep, 2007 | Pubmed ID: 17643108 Protein Mobility and Diffusive Barriers in Escherichia Coli: Consequences of Osmotic Stress Molecular Microbiology. May, 2007 | Pubmed ID: 17462029 The Lipid Dependence of Melittin Action Investigated by Dual-color Fluorescence Burst Analysis Biophysical Journal. Jul, 2007 | Pubmed ID: 17434946 Biochemistry. A Missing Link in Membrane Protein Evolution Science (New York, N.Y.). Mar, 2007 | Pubmed ID: 17332400 Dual-color Fluorescence-burst Analysis to Probe Protein Efflux Through the Mechanosensitive Channel MscL Biophysical Journal. Feb, 2007 | Pubmed ID: 17142294 On the Decrease in Lateral Mobility of Phospholipids by Sugars Biophysical Journal. Mar, 2007 | Pubmed ID: 17142271 Ion Specificity and Ionic Strength Dependence of the Osmoregulatory ABC Transporter OpuA The Journal of Biological Chemistry. Oct, 2006 | Pubmed ID: 16844687 The Oligomeric State and Stability of the Mannitol Transporter, EnzymeII(mtl), from Escherichia Coli: a Fluorescence Correlation Spectroscopy Study Protein Science : a Publication of the Protein Society. Aug, 2006 | Pubmed ID: 16823033 A Sensor for Intracellular Ionic Strength Proceedings of the National Academy of Sciences of the United States of America. Jul, 2006 | Pubmed ID: 16815971 The Riboflavin Transporter RibU in Lactococcus Lactis: Molecular Characterization of Gene Expression and the Transport Mechanism Journal of Bacteriology. Apr, 2006 | Pubmed ID: 16585736 ABC Transporter Architecture and Regulatory Roles of Accessory Domains FEBS Letters. Feb, 2006 | Pubmed ID: 16375896 Functional Analysis of Detergent-solubilized and Membrane-reconstituted ATP-binding Cassette Transporters Methods in Enzymology. 2005 | Pubmed ID: 16399364 Engineering Covalent Oligomers of the Mechanosensitive Channel of Large Conductance from Escherichia Coli with Native Conductance and Gating Characteristics Protein Science : a Publication of the Protein Society. Dec, 2005 | Pubmed ID: 16322576 The Activity of the Lactose Transporter from Streptococcus Thermophilus is Increased by Phosphorylated IIA and the Action of Beta-galactosidase Biochemistry. Dec, 2005 | Pubmed ID: 16313191 Substrate-induced Conformational Changes in the Membrane-embedded IIC(mtl)-domain of the Mannitol Permease from Escherichia Coli, EnzymeII(mtl), Probed by Tryptophan Phosphorescence Spectroscopy The Journal of Biological Chemistry. Oct, 2005 | Pubmed ID: 16093245 Specificity and Selectivity Determinants of Peptide Transport in Lactococcus Lactis and Other Microorganisms Molecular Microbiology. Aug, 2005 | Pubmed ID: 16045610 Functional Interactions Between the Subunits of the Lactose Transporter from Streptococcus Thermophilus Journal of Molecular Biology. Jul, 2005 | Pubmed ID: 15919090 Stoichiometry and Substrate Affinity of the Mannitol Transporter, EnzymeIImtl, from Escherichia Coli Biophysical Journal. Jul, 2005 | Pubmed ID: 15879478 Substrate Specificity and Ionic Regulation of GlnPQ from Lactococcus Lactis. An ATP-binding Cassette Transporter with Four Extracytoplasmic Substrate-binding Domains The Journal of Biological Chemistry. Jun, 2005 | Pubmed ID: 15795228 The First Cytoplasmic Loop of the Mannitol Permease from Escherichia Coli is Accessible for Sulfhydryl Reagents from the Periplasmic Side of the Membrane Journal of Molecular Biology. Feb, 2005 | Pubmed ID: 15713459 Lactococcus Lactis Uses MscL As Its Principal Mechanosensitive Channel The Journal of Biological Chemistry. Mar, 2005 | Pubmed ID: 15613476 Distribution, Lateral Mobility and Function of Membrane Proteins Incorporated into Giant Unilamellar Vesicles Biophysical Journal. Feb, 2005 | Pubmed ID: 15574707 Two Homologous Oligopeptide Binding Protein Genes (oppA) in Lactococcus Lactis Opp2 [corrected] International Journal of Food Microbiology. Dec, 2004 | Pubmed ID: 15527913 Bacterial Osmosensing: Roles of Membrane Structure and Electrostatics in Lipid-protein and Protein-protein Interactions Biochimica Et Biophysica Acta. Nov, 2004 | Pubmed ID: 15519310 Lipid-mediated Light Activation of a Mechanosensitive Channel of Large Conductance Langmuir : the ACS Journal of Surfaces and Colloids. Aug, 2004 | Pubmed ID: 15301476 The Binding Specificity of OppA Determines the Selectivity of the Oligopeptide ATP-binding Cassette Transporter The Journal of Biological Chemistry. Jul, 2004 | Pubmed ID: 15169767 Evaluation of the Flow-dialysis Technique for Analysis of Protein-ligand Interactions: an Experimental and a Monte Carlo Study Biophysical Journal. Apr, 2004 | Pubmed ID: 15041640 On the Role of the Two Extracytoplasmic Substrate-binding Domains in the ABC Transporter OpuA The EMBO Journal. Nov, 2003 | Pubmed ID: 14609945 Identification of the Dimer Interface of the Lactose Transport Protein from Streptococcus Thermophilus Journal of Molecular Biology. Oct, 2003 | Pubmed ID: 14499618 The ATP/substrate Stoichiometry of the ATP-binding Cassette (ABC) Transporter OpuA The Journal of Biological Chemistry. Aug, 2003 | Pubmed ID: 12766159 ABC Transporters: One, Two or Four Extracytoplasmic Substrate-binding Sites? EMBO Reports. Oct, 2002 | Pubmed ID: 12370206 Transporters and Their Roles in LAB Cell Physiology Antonie Van Leeuwenhoek. Aug, 2002 | Pubmed ID: 12369186 Combined In-gel Tryptic Digestion and CNBr Cleavage for the Generation of Peptide Maps of an Integral Membrane Protein with MALDI-TOF Mass Spectrometry Biochimica Et Biophysica Acta. Sep, 2002 | Pubmed ID: 12206901 Spatial Organization of Bacteriorhodopsin in Model Membranes. Light-induced Mobility Changes The Journal of Biological Chemistry. Oct, 2002 | Pubmed ID: 12167614 Quaternary Structure and Function of Transport Proteins Trends in Biochemical Sciences. May, 2002 | Pubmed ID: 12076536 How Do Membrane Proteins Sense Water Stress? Molecular Microbiology. May, 2002 | Pubmed ID: 12010487 Oligomeric State of Membrane Transport Proteins Analyzed with Blue Native Electrophoresis and Analytical Ultracentrifugation Journal of Molecular Biology. Apr, 2002 | Pubmed ID: 11955011 Mapping of the Dimer Interface of the Escherichia Coli Mannitol Permease by Cysteine Cross-linking The Journal of Biological Chemistry. Apr, 2002 | Pubmed ID: 11854301 On the Osmotic Signal and Osmosensing Mechanism of an ABC Transport System for Glycine Betaine The EMBO Journal. Dec, 2001 | Pubmed ID: 11742979 The Lactose Transport Protein is a Cooperative Dimer with Two Sugar Translocation Pathways The EMBO Journal. Jun, 2001 | Pubmed ID: 11406582 Combinatorial Peptide Libraries Reveal the Ligand-binding Mechanism of the Oligopeptide Receptor OppA of Lactococcus Lactis Proceedings of the National Academy of Sciences of the United States of America. Nov, 2000 | Pubmed ID: 11050157 On the Binding Mechanism of the Peptide Receptor of the Oligopeptide Transport System of Lactococcus Lactis The EMBO Journal. Jul, 2000 | Pubmed ID: 10899119 Osmoregulated ABC-transport System of Lactococcus Lactis Senses Water Stress Via Changes in the Physical State of the Membrane Proceedings of the National Academy of Sciences of the United States of America. Jun, 2000 | Pubmed ID: 10860977 Kinetics and Substrate Specificity of Membrane-reconstituted Peptide Transporter DtpT of Lactococcus Lactis Journal of Bacteriology. May, 2000 | Pubmed ID: 10762255 Specificity Mutants of the Binding Protein of the Oligopeptide Transport System of Lactococcus Lactis Journal of Bacteriology. Mar, 2000 | Pubmed ID: 10692365 Glycine Betaine Transport in Lactococcus Lactis is Osmotically Regulated at the Level of Expression and Translocation Activity Journal of Bacteriology. Jan, 2000 | Pubmed ID: 10613881 Phosphorylation and Functional Properties of the IIA Domain of the Lactose Transport Protein of Streptococcus Thermophilus Journal of Bacteriology. Jan, 1999 | Pubmed ID: 9882680 Mechanism of Osmotic Activation of the Quaternary Ammonium Compound Transporter (QacT) of Lactobacillus Plantarum Journal of Bacteriology. Nov, 1998 | Pubmed ID: 9791101 Physiological Response of Lactobacillus Plantarum to Salt and Nonelectrolyte Stress Journal of Bacteriology. Sep, 1998 | Pubmed ID: 9721316 Author Spotlight: Tackling Challenges in Synthetic Cell Engineering Jelmer Coenradij1, Eleonora Bailoni1, Bert Poolman1 1Department of Biochemistry, University of Groningen JoVE 66627 Biochemistry
Author Spotlight: Tackling Challenges in Synthetic Cell Engineering Jelmer Coenradij1, Eleonora Bailoni1, Bert Poolman1 1Department of Biochemistry, University of Groningen JoVE 66627 Biochemistry
