measurement-scattering-nonlinearities-from-single-plasmonic

Measurement of Scattering Nonlinearities from a Single Plasmonic Nanoparticle

Plasmonics, which are based on the collective oscillation of electrons due to light excitation, involve strongly enhanced local electric fields and thus ...

National Taiwan University

To construct the recombinant plasmid pPHU281_A_Spec_B, which knock out Porphyrmonas gingivalis (Pg) FimA gene.

[Construction of recombinant plasmid with Porphyromonas gingivalis FimA deficiency].

To construct a recombinant plasmid containing the upstream of fimC and downstream of fimE of Porphyromonas gingivalis, designated as pPHU281-C-Spec-E, which may be further used to knock out fimCDE gene to determine the role of FimCDE in the infection by P. gingivalis.

[Construction of recombinant for generation of Porphyromonas gingivalis minor accessory proteins FimCDE deficient strain].

Nonlinear plasmonics has attracted a lot of interests due to its wide applications. Recently, we demonstrated saturation and reverse saturation of scattering from a single plasmonic nanoparticle, which exhibits extremely narrow side lobes and central peaks in scattering images [ACS Photonics 1(1), 32 (2014)]. It is desirable to extract the reversed saturated part to further enhance optical resolution. However, such separation is not possible with conventional confocal microscope. Here we combine reverse saturable scattering and saturated excitation (SAX) microscopy. With quantitative analyses of amplitude and phase of SAX signals, unexpectedly high-order nonlinearities are revealed. Our result provides greatly reduced width in point spread function of scattering-based optical microscopy. It will find applications in not only nonlinear material analysis, but also high-resolution biomedical microscopy.

Article	Year
[Construction of recombinant plasmid with Porphyromonas gingivalis FimA deficiency]. Zhonghua kou qiang yi xue za zhi = Zhonghua kouqiang yixue zazhi = Chinese journal of stomatology\| PubMed ID: 23302429	2012
[Construction of recombinant for generation of Porphyromonas gingivalis minor accessory proteins FimCDE deficient strain]. Shanghai kou qiang yi xue = Shanghai journal of stomatology\| PubMed ID: 24469122	2013
Point spread function analysis with saturable and reverse saturable scattering. Optics express\| PubMed ID: 25401635	2014

Kuan-Yu Li

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