measuring-dna-damage-repair-mouse-splenocytes-after-chronic-vivo

Measuring DNA Damage and Repair in Mouse Splenocytes After Chronic <em>In Vivo</em> Exposure to Very Low Doses of Beta- and Gamma-Radiation

Low dose radiation exposure may produce a variety of biological effects that are different in quantity and quality from the effects produced by high ...

Canadian Nuclear Laboratories

Anesthesia monitoring equipment for laboratory animals.

In this article we discuss the importance of monitoring body temperature. Some effects of hypothermia are covered, along with the benefits of careful monitoring. Also discussed are the basic use and application of the most common monitoring methods, as well as the methods we used to help maintain body temperature.

Principles of anesthesia monitoring--body temperature.

Principles of anesthesia monitoring--respiration.

Principles of anesthesia monitoring--arterial blood pressure.

Principles of anesthesia monitoring and electrocardiogram.

Principles of anesthesia monitoring-capnography.

Health effects of tritium, a β-emitter and a by-product of the nuclear industry, is a subject of significant controversy. This mouse in vivo study was undertaken to monitor biological effects of low level tritium exposure. Mice were exposed to tritiated drinking water (HTO) at 10 KBq/L, 1 MBq/L and 20 MBq/L concentrations for one month. The treatment did not result in a significant increase of apoptosis in splenocytes. To examine if this low level tritium exposure alters radiosensitivity, the extracted splenocytes were challenged in vitro with 2 Gy γ-radiation, and apoptotic responses at 1 and 24 h were measured. No alterations in the radiosensitivity were detected in cells from mice exposed to tritium compared to sham-treated mice. In contrast, low dose γ-irradiation at 20 or 100 mGy, resulted in a significant increase in resistance to apoptotic cell death after 2 Gy irradiation; an indication of the radioadaptive response. Overall, our data suggest that low concentrations of tritium given to mice as HTO in drinking water do not exert cytotoxic effect in splenocytes, nor do they change cellular sensitivity to additional high dose γ-radiation. The latter may be considered as the lack of a radioadaptive response, typically observed after low dose γ-irradiation.

The lack of cytotoxic effect and radioadaptive response in splenocytes of mice exposed to low level internal β-particle irradiation through tritiated drinking water in vivo.

Article	Year
Anesthesia monitoring equipment for laboratory animals. Lab animal\| PubMed ID: 15224116	2004
Principles of anesthesia monitoring--body temperature. Journal of investigative surgery : the official journal of the Academy of Surgical Research\| PubMed ID: 19160148	2008
Principles of anesthesia monitoring--respiration. Journal of investigative surgery : the official journal of the Academy of Surgical Research\| PubMed ID: 19191161	2009
Principles of anesthesia monitoring--arterial blood pressure. Journal of investigative surgery : the official journal of the Academy of Surgical Research\| PubMed ID: 19283619	2009
Principles of anesthesia monitoring and electrocardiogram. Journal of investigative surgery : the official journal of the Academy of Surgical Research\| PubMed ID: 19842908	2009
Principles of anesthesia monitoring-capnography. Journal of investigative surgery : the official journal of the Academy of Surgical Research\| PubMed ID: 20001816	2009
The lack of cytotoxic effect and radioadaptive response in splenocytes of mice exposed to low level internal β-particle irradiation through tritiated drinking water in vivo. International journal of molecular sciences\| PubMed ID: 24317437	2013

Matthew Flegal

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