Stuart F.J. Le Grice RT Biochemistry Section, HIV Drug Resistance Program Frederick National Laboratory for Cancer Research Biography Publications Institution JoVE Articles Stuart F.J. Le Grice has not added a biography. If you are Stuart F.J. Le Grice and would like to personalize this page please email our Author Liaison for assistance. Publications The HIV-2 Rev-response Element: Determining Secondary Structure and Defining Folding Intermediates Nucleic Acids Research. May, 2013 | Pubmed ID: 23640333 Examining the Role of the HIV-1 Reverse Transcriptase P51 Subunit in Positioning and Hydrolysis of RNA/DNA Hybrids The Journal of Biological Chemistry. Apr, 2013 | Pubmed ID: 23595992 Structural Complexity of Dengue Virus Untranslated Regions: Cis-acting RNA Motifs and Pseudoknot Interactions Modulating Functionality of the Viral Genome Nucleic Acids Research. May, 2013 | Pubmed ID: 23531545 A Cis-acting Element in Retroviral Genomic RNA Links Gag-Pol Ribosomal Frameshifting to Selective Viral RNA Encapsidation Cell Host & Microbe. Feb, 2013 | Pubmed ID: 23414758 Structural Analysis of Monomeric Retroviral Reverse Transcriptase in Complex with an RNA/DNA Hybrid Nucleic Acids Research. Apr, 2013 | Pubmed ID: 23382176 Complexes of HIV-1 RT, NNRTI and RNA/DNA Hybrid Reveal a Structure Compatible with RNA Degradation Nature Structural & Molecular Biology. Feb, 2013 | Pubmed ID: 23314251 探索 Ty1 反转录转座子 RNA 结构内病毒样颗粒。 Nucleic Acids Research. Oct, 2012 | Pubmed ID: 23093595 Human Immunodeficiency Virus Reverse Transcriptase: 25 Years of Research, Drug Discovery, and Promise The Journal of Biological Chemistry. Nov, 2012 | Pubmed ID: 23043108 Viral Reverse Transcriptases Show Selective High Affinity Binding to DNA-DNA Primer-templates That Resemble the Polypurine Tract PloS One. 2012 | Pubmed ID: 22848574 Probing Retroviral and Retrotransposon Genome Structures: The "SHAPE" of Things to Come Molecular Biology International. 2012 | Pubmed ID: 22685659 Multimode, Cooperative Mechanism of Action of Allosteric HIV-1 Integrase Inhibitors The Journal of Biological Chemistry. May, 2012 | Pubmed ID: 22437836 Crystal Structures of the Reverse Transcriptase-associated Ribonuclease H Domain of Xenotropic Murine Leukemia-virus Related Virus Journal of Structural Biology. Mar, 2012 | Pubmed ID: 22366278 Multiple Sources of Contamination in Samples from Patients Reported to Have XMRV Infection PloS One. 2012 | Pubmed ID: 22363509 Intrinsic DNA Synthesis Fidelity of Xenotropic Murine Leukemia Virus-related Virus Reverse Transcriptase The FEBS Journal. Apr, 2012 | Pubmed ID: 22340433 Mutagenesis of Human Immunodeficiency Virus Reverse Transcriptase P51 Subunit Defines Residues Contributing to Vinylogous Urea Inhibition of Ribonuclease H Activity The Journal of Biological Chemistry. Feb, 2012 | Pubmed ID: 22105069 Correlating SHAPE Signatures with Three-dimensional RNA Structures RNA (New York, N.Y.). Sep, 2011 | Pubmed ID: 21752927 Inhibition of Multi-drug Resistant HIV-1 Reverse Transcriptase by Nucleoside β-triphosphates Bioorganic & Medicinal Chemistry Letters. Jun, 2011 | Pubmed ID: 21605974 Synthesis, Activity, and Structural Analysis of Novel α-hydroxytropolone Inhibitors of Human Immunodeficiency Virus Reverse Transcriptase-associated Ribonuclease H Journal of Medicinal Chemistry. Jul, 2011 | Pubmed ID: 21568335 SHAPE Analysis of the FIV Leader RNA Reveals a Structural Switch Potentially Controlling Viral Packaging and Genome Dimerization Nucleic Acids Research. Aug, 2011 | Pubmed ID: 21546549 New Monoterpene Glycosides and Phenolic Compounds from Distylium Racemosum and Their Inhibitory Activity Against Ribonuclease H Bioorganic & Medicinal Chemistry Letters. May, 2011 | Pubmed ID: 21489793 The Xenotropic Murine Leukemia Virus-related Retrovirus Debate Continues at First International Workshop Retrovirology. 2010 | Pubmed ID: 21176195 Initiation Complex Dynamics Direct the Transitions Between Distinct Phases of Early HIV Reverse Transcription Nature Structural & Molecular Biology. Dec, 2010 | Pubmed ID: 21102446 The RNA Transport Element of the Murine MusD Retrotransposon Requires Long-range Intramolecular Interactions for Function The Journal of Biological Chemistry. Dec, 2010 | Pubmed ID: 20978285 Structure-activity Analysis of Vinylogous Urea Inhibitors of Human Immunodeficiency Virus-encoded Ribonuclease H Antimicrobial Agents and Chemotherapy. Sep, 2010 | Pubmed ID: 20547794 Solid-Phase Synthesis of 5'-O-β,γ-Methylenetriphosphate Derivatives of Nucleosides and Evaluation of Their Inhibitory Activity Against HIV-1 Reverse Transcriptase Tetrahedron Letters. Jun, 2010 | Pubmed ID: 20454539 HIV-1 Transmission by Dendritic Cell-specific ICAM-3-grabbing Nonintegrin (DC-SIGN) is Regulated by Determinants in the Carbohydrate Recognition Domain That Are Absent in Liver/lymph Node-SIGN (L-SIGN) The Journal of Biological Chemistry. Jan, 2010 | Pubmed ID: 19833723 Reverse Transcriptase in Motion: Conformational Dynamics of Enzyme-substrate Interactions Biochimica Et Biophysica Acta. May, 2010 | Pubmed ID: 19665597 Revisiting Plus-strand DNA Synthesis in Retroviruses and Long Terminal Repeat Retrotransposons: Dynamics of Enzyme: Substrate Interactions Viruses. Dec, 2009 | Pubmed ID: 21994564 SHAMS: Combining Chemical Modification of RNA with Mass Spectrometry to Examine Polypurine Tract-containing RNA/DNA Hybrids RNA (New York, N.Y.). Aug, 2009 | Pubmed ID: 19535461 Probing Anomalous Structural Features in Polypurine Tract-containing RNA-DNA Hybrids with Neomycin B Biochemistry. Jul, 2009 | Pubmed ID: 19449839 Identifying and Characterizing a Functional HIV-1 Reverse Transcriptase-binding Site on Integrase The Journal of Biological Chemistry. Mar, 2009 | Pubmed ID: 19150986 Dissecting APOBEC3G Substrate Specificity by Nucleoside Analog Interference The Journal of Biological Chemistry. Mar, 2009 | Pubmed ID: 19136562 Slide into Action: Dynamic Shuttling of HIV Reverse Transcriptase on Nucleic Acid Substrates Science (New York, N.Y.). Nov, 2008 | Pubmed ID: 19008444 Structural Analysis of Protein-RNA Interactions with Mass Spectrometry Methods in Molecular Biology (Clifton, N.J.). 2008 | Pubmed ID: 18982294 Examining the Ribonuclease H Primer Grip of HIV-1 Reverse Transcriptase by Charge Neutralization of RNA/DNA Hybrids Nucleic Acids Research. Nov, 2008 | Pubmed ID: 18836193 Resistance to RevM10 Inhibition Reflects a Conformational Switch in the HIV-1 Rev Response Element Proceedings of the National Academy of Sciences of the United States of America. Sep, 2008 | Pubmed ID: 18776047 HIV-1 Ribonuclease H Inhibitory Phenolic Glycosides from Eugenia Hyemalis Journal of Natural Products. Sep, 2008 | Pubmed ID: 18763827 Mutations M184V and Y115F in HIV-1 Reverse Transcriptase Discriminate Against "nucleotide-competing Reverse Transcriptase Inhibitors" The Journal of Biological Chemistry. Oct, 2008 | Pubmed ID: 18728003 Dynamic Binding Orientations Direct Activity of HIV Reverse Transcriptase Nature. May, 2008 | Pubmed ID: 18464735 Structural Probing of the HIV-1 Polypurine Tract RNA:DNA Hybrid Using Classic Nucleic Acid Ligands Nucleic Acids Research. May, 2008 | Pubmed ID: 18400780 High-resolution NMR Analysis of the Conformations of Native and Base Analog Substituted Retroviral and LTR-retrotransposon PPT Primers Chemistry & Biology. Mar, 2008 | Pubmed ID: 18355725 An HIV RNase H Inhibitory 1,3,4,5-tetragalloylapiitol from the African Plant Hylodendron Gabunensis Journal of Natural Products. Oct, 2007 | Pubmed ID: 17935297 A Dimeric Lactone from Ardisia Japonica with Inhibitory Activity for HIV-1 and HIV-2 Ribonuclease H Journal of Natural Products. May, 2007 | Pubmed ID: 17397219 Investigating the "steric Gate" of Human Immunodeficiency Virus Type 1 (HIV-1) Reverse Transcriptase by Targeted Insertion of Unnatural Amino Acids Biochemistry. Feb, 2007 | Pubmed ID: 17274599 Exploiting Structurally Diverse Nucleoside Analogs As Probes of Reverse Transcription Complexes Current HIV Research. Jan, 2007 | Pubmed ID: 17266554 Purine Analog Substitution of the HIV-1 Polypurine Tract Primer Defines Regions Controlling Initiation of Plus-strand DNA Synthesis Nucleic Acids Research. 2007 | Pubmed ID: 17164285 Examining Interactions of HIV-1 Reverse Transcriptase with Single-stranded Template Nucleotides by Nucleoside Analog Interference The Journal of Biological Chemistry. Sep, 2006 | Pubmed ID: 16867979 Analysis of HIV-1 Replication Block Due to Substitutions at F61 Residue of Reverse Transcriptase Reveals Additional Defects Involving the RNase H Function Nucleic Acids Research. 2006 | Pubmed ID: 16723431 Nucleotide Excision Repair and Template-independent Addition by HIV-1 Reverse Transcriptase in the Presence of Nucleocapsid Protein The Journal of Biological Chemistry. Apr, 2006 | Pubmed ID: 16500895 Examining Ty3 Polypurine Tract Structure and Function by Nucleoside Analog Interference The Journal of Biological Chemistry. Feb, 2006 | Pubmed ID: 16306041 A Ribose Sugar Conformational Switch in the LTR-retrotransposon Ty3 Polypurine Tract-containing RNA/DNA Hybrid Journal of the American Chemical Society. Nov, 2005 | Pubmed ID: 16305191 Interaction of the Ty3 Reverse Transcriptase Thumb Subdomain with Template-primer The Journal of Biological Chemistry. Aug, 2005 | Pubmed ID: 15944162 Attenuation of DNA Replication by HIV-1 Reverse Transcriptase Near the Central Termination Sequence Biochemistry. Apr, 2005 | Pubmed ID: 15807528 Investigating HIV-1 Polypurine Tract Geometry Via Targeted Insertion of Abasic Lesions in the (-)-DNA Template and (+)-RNA Primer The Journal of Biological Chemistry. May, 2005 | Pubmed ID: 15778225 Selective Inhibition of HIV-1 Reverse Transcriptase-associated Ribonuclease H Activity by Hydroxylated Tropolones Nucleic Acids Research. 2005 | Pubmed ID: 15741178 Functional Roles of Carboxylate Residues Comprising the DNA Polymerase Active Site Triad of Ty3 Reverse Transcriptase Nucleic Acids Research. 2005 | Pubmed ID: 15647500 Mass Spectrometric Analysis of the HIV-1 Integrase-pyridoxal 5'-phosphate Complex Reveals a New Binding Site for a Nucleotide Inhibitor The Journal of Biological Chemistry. Mar, 2005 | Pubmed ID: 15615720 Site- and Subunit-specific Incorporation of Unnatural Amino Acids into HIV-1 Reverse Transcriptase Protein Expression and Purification. Nov, 2004 | Pubmed ID: 15477080 Synthetic TRNALys,3 As the Replication Primer for the HIV-1HXB2 and HIV-1Mal Genomes Nucleic Acids Research. 2004 | Pubmed ID: 15342789 A Capillary Electrophoretic Assay for Ribonuclease H Activity Analytical Biochemistry. Aug, 2004 | Pubmed ID: 15265735 Two Modes of HIV-1 Polypurine Tract Cleavage Are Affected by Introducing Locked Nucleic Acid Analogs into the (-) DNA Template The Journal of Biological Chemistry. Aug, 2004 | Pubmed ID: 15220330 'Binding, Bending and Bonding': Polypurine Tract-primed Initiation of Plus-strand DNA Synthesis in Human Immunodeficiency Virus The International Journal of Biochemistry & Cell Biology. Sep, 2004 | Pubmed ID: 15183342 Identification of an Inhibitor-binding Site to HIV-1 Integrase with Affinity Acetylation and Mass Spectrometry Proceedings of the National Academy of Sciences of the United States of America. May, 2004 | Pubmed ID: 15118107 Using Pyrrolo-deoxycytosine to Probe RNA/DNA Hybrids Containing the Human Immunodeficiency Virus Type-1 3' Polypurine Tract Nucleic Acids Research. 2004 | Pubmed ID: 15004241 A Fluorescence-based High-throughput Screening Assay for Inhibitors of Human Immunodeficiency Virus-1 Reverse Transcriptase-associated Ribonuclease H Activity Analytical Biochemistry. Nov, 2003 | Pubmed ID: 14705777 "In the Beginning": Initiation of Minus Strand DNA Synthesis in Retroviruses and LTR-containing Retrotransposons Biochemistry. Dec, 2003 | Pubmed ID: 14661945 Hydrolysis of RNA/DNA Hybrids Containing Nonpolar Pyrimidine Isosteres Defines Regions Essential for HIV Type 1 Polypurine Tract Selection Proceedings of the National Academy of Sciences of the United States of America. Sep, 2003 | Pubmed ID: 12972638 Human Immunodeficiency Virus Type 2 Reverse Transcriptase Activity in Model Systems That Mimic Steps in Reverse Transcription Journal of Virology. Jul, 2003 | Pubmed ID: 12805462 Role of the Reverse Transcriptase, Nucleocapsid Protein, and Template Structure in the Two-step Transfer Mechanism in Retroviral Recombination The Journal of Biological Chemistry. Aug, 2003 | Pubmed ID: 12801926 Effects of TRNA 3 Lys Aminoacylation on the Initiation of HIV-1 Reverse Transcription Biochimie. May, 2003 | Pubmed ID: 12763311 Nonpolar Thymine Isosteres in the Ty3 Polypurine Tract DNA Template Modulate Processing and Provide a Model for Its Recognition by Ty3 Reverse Transcriptase The Journal of Biological Chemistry. Jul, 2003 | Pubmed ID: 12730227 Uncovering the Complexities of Retroviral Ribonuclease H Reveals Its Potential As a Therapeutic Target AIDS Reviews. Oct-Dec, 2002 | Pubmed ID: 12555693 Identification of Specific HIV-1 Reverse Transcriptase Contacts to the Viral RNA:tRNA Complex by Mass Spectrometry and a Primary Amine Selective Reagent Proceedings of the National Academy of Sciences of the United States of America. Dec, 2002 | Pubmed ID: 12461175 Mutations in the Ribonuclease H Active Site of HIV-RT Reveal a Role for This Site in Stabilizing Enzyme-primer-template Binding Biochemistry. Sep, 2002 | Pubmed ID: 12206668 Direct and Indirect Contributions of RNA Secondary Structure Elements to the Initiation of HIV-1 Reverse Transcription The Journal of Biological Chemistry. Nov, 2002 | Pubmed ID: 12194974 Functional Interactions of Nucleocapsid Protein of Feline Immunodeficiency Virus and Cellular Prion Protein with the Viral RNA Journal of Molecular Biology. Apr, 2002 | Pubmed ID: 12054775 Mutating Conserved Residues in the Ribonuclease H Domain of Ty3 Reverse Transcriptase Affects Specialized Cleavage Events The Journal of Biological Chemistry. Jul, 2002 | Pubmed ID: 11994277 Altering the RNase H Primer Grip of Human Immunodeficiency Virus Reverse Transcriptase Modifies Cleavage Specificity Biochemistry. Apr, 2002 | Pubmed ID: 11939780 Pre-existing Distortions in Nucleic Acid Structure Aid Polypurine Tract Selection by HIV-1 Reverse Transcriptase The Journal of Biological Chemistry. May, 2002 | Pubmed ID: 11875059 Structural and Functional Properties of the HIV-1 RNA-tRNA(Lys)3 Primer Complex Annealed by the Nucleocapsid Protein: Comparison with the Heat-annealed Complex RNA (New York, N.Y.). Jan, 2002 | Pubmed ID: 11873759 Ty3 Integrase is Required for Initiation of Reverse Transcription Journal of Virology. Mar, 2002 | Pubmed ID: 11861848 使用高通量形状的RNA二级结构预测 Sabrina Lusvarghi*1, Joanna Sztuba-Solinska*1, Katarzyna J. Purzycka1, Jason W. Rausch1, Stuart F.J. Le Grice1 1RT Biochemistry Section, HIV Drug Resistance Program, Frederick National Laboratory for Cancer Research JoVE 50243 Biology
使用高通量形状的RNA二级结构预测 Sabrina Lusvarghi*1, Joanna Sztuba-Solinska*1, Katarzyna J. Purzycka1, Jason W. Rausch1, Stuart F.J. Le Grice1 1RT Biochemistry Section, HIV Drug Resistance Program, Frederick National Laboratory for Cancer Research JoVE 50243 Biology