Stuart F.J. Le Grice RT Biochemistry Section, HIV Drug Resistance Program Frederick National Laboratory for Cancer Research Biography Publications Institution JoVE Articles Stuart F.J. Le Grice has not added a biography. If you are Stuart F.J. Le Grice and would like to personalize this page please email our Author Liaison for assistance. Publications The HIV-2 Rev-response Element: Determining Secondary Structure and Defining Folding Intermediates Nucleic Acids Research. May, 2013 | Pubmed ID: 23640333 Examining the Role of the HIV-1 Reverse Transcriptase P51 Subunit in Positioning and Hydrolysis of RNA/DNA Hybrids The Journal of Biological Chemistry. Apr, 2013 | Pubmed ID: 23595992 Structural Complexity of Dengue Virus Untranslated Regions: Cis-acting RNA Motifs and Pseudoknot Interactions Modulating Functionality of the Viral Genome Nucleic Acids Research. May, 2013 | Pubmed ID: 23531545 A Cis-acting Element in Retroviral Genomic RNA Links Gag-Pol Ribosomal Frameshifting to Selective Viral RNA Encapsidation Cell Host & Microbe. Feb, 2013 | Pubmed ID: 23414758 Structural Analysis of Monomeric Retroviral Reverse Transcriptase in Complex with an RNA/DNA Hybrid Nucleic Acids Research. Apr, 2013 | Pubmed ID: 23382176 Complexes of HIV-1 RT, NNRTI and RNA/DNA Hybrid Reveal a Structure Compatible with RNA Degradation Nature Structural & Molecular Biology. Feb, 2013 | Pubmed ID: 23314251 Exploring Ty1 Retrotransposon RNA Structure Within Virus-like Particles Nucleic Acids Research. Oct, 2012 | Pubmed ID: 23093595 Human Immunodeficiency Virus Reverse Transcriptase: 25 Years of Research, Drug Discovery, and Promise The Journal of Biological Chemistry. Nov, 2012 | Pubmed ID: 23043108 Viral Reverse Transcriptases Show Selective High Affinity Binding to DNA-DNA Primer-templates That Resemble the Polypurine Tract PloS One. 2012 | Pubmed ID: 22848574 Probing Retroviral and Retrotransposon Genome Structures: The "SHAPE" of Things to Come Molecular Biology International. 2012 | Pubmed ID: 22685659 Multimode, Cooperative Mechanism of Action of Allosteric HIV-1 Integrase Inhibitors The Journal of Biological Chemistry. May, 2012 | Pubmed ID: 22437836 Crystal Structures of the Reverse Transcriptase-associated Ribonuclease H Domain of Xenotropic Murine Leukemia-virus Related Virus Journal of Structural Biology. Mar, 2012 | Pubmed ID: 22366278 Multiple Sources of Contamination in Samples from Patients Reported to Have XMRV Infection PloS One. 2012 | Pubmed ID: 22363509 Intrinsic DNA Synthesis Fidelity of Xenotropic Murine Leukemia Virus-related Virus Reverse Transcriptase The FEBS Journal. Apr, 2012 | Pubmed ID: 22340433 Mutagenesis of Human Immunodeficiency Virus Reverse Transcriptase P51 Subunit Defines Residues Contributing to Vinylogous Urea Inhibition of Ribonuclease H Activity The Journal of Biological Chemistry. Feb, 2012 | Pubmed ID: 22105069 Correlating SHAPE Signatures with Three-dimensional RNA Structures RNA (New York, N.Y.). Sep, 2011 | Pubmed ID: 21752927 Inhibition of Multi-drug Resistant HIV-1 Reverse Transcriptase by Nucleoside β-triphosphates Bioorganic & Medicinal Chemistry Letters. Jun, 2011 | Pubmed ID: 21605974 Synthesis, Activity, and Structural Analysis of Novel α-hydroxytropolone Inhibitors of Human Immunodeficiency Virus Reverse Transcriptase-associated Ribonuclease H Journal of Medicinal Chemistry. Jul, 2011 | Pubmed ID: 21568335 SHAPE Analysis of the FIV Leader RNA Reveals a Structural Switch Potentially Controlling Viral Packaging and Genome Dimerization Nucleic Acids Research. Aug, 2011 | Pubmed ID: 21546549 New Monoterpene Glycosides and Phenolic Compounds from Distylium Racemosum and Their Inhibitory Activity Against Ribonuclease H Bioorganic & Medicinal Chemistry Letters. May, 2011 | Pubmed ID: 21489793 The Xenotropic Murine Leukemia Virus-related Retrovirus Debate Continues at First International Workshop Retrovirology. 2010 | Pubmed ID: 21176195 Initiation Complex Dynamics Direct the Transitions Between Distinct Phases of Early HIV Reverse Transcription Nature Structural & Molecular Biology. Dec, 2010 | Pubmed ID: 21102446 The RNA Transport Element of the Murine MusD Retrotransposon Requires Long-range Intramolecular Interactions for Function The Journal of Biological Chemistry. Dec, 2010 | Pubmed ID: 20978285 Structure-activity Analysis of Vinylogous Urea Inhibitors of Human Immunodeficiency Virus-encoded Ribonuclease H Antimicrobial Agents and Chemotherapy. Sep, 2010 | Pubmed ID: 20547794 Solid-Phase Synthesis of 5'-O-β,γ-Methylenetriphosphate Derivatives of Nucleosides and Evaluation of Their Inhibitory Activity Against HIV-1 Reverse Transcriptase Tetrahedron Letters. Jun, 2010 | Pubmed ID: 20454539 HIV-1 Transmission by Dendritic Cell-specific ICAM-3-grabbing Nonintegrin (DC-SIGN) is Regulated by Determinants in the Carbohydrate Recognition Domain That Are Absent in Liver/lymph Node-SIGN (L-SIGN) The Journal of Biological Chemistry. Jan, 2010 | Pubmed ID: 19833723 Reverse Transcriptase in Motion: Conformational Dynamics of Enzyme-substrate Interactions Biochimica Et Biophysica Acta. May, 2010 | Pubmed ID: 19665597 Revisiting Plus-strand DNA Synthesis in Retroviruses and Long Terminal Repeat Retrotransposons: Dynamics of Enzyme: Substrate Interactions Viruses. Dec, 2009 | Pubmed ID: 21994564 SHAMS: Combining Chemical Modification of RNA with Mass Spectrometry to Examine Polypurine Tract-containing RNA/DNA Hybrids RNA (New York, N.Y.). Aug, 2009 | Pubmed ID: 19535461 Probing Anomalous Structural Features in Polypurine Tract-containing RNA-DNA Hybrids with Neomycin B Biochemistry. Jul, 2009 | Pubmed ID: 19449839 Identifying and Characterizing a Functional HIV-1 Reverse Transcriptase-binding Site on Integrase The Journal of Biological Chemistry. Mar, 2009 | Pubmed ID: 19150986 Dissecting APOBEC3G Substrate Specificity by Nucleoside Analog Interference The Journal of Biological Chemistry. Mar, 2009 | Pubmed ID: 19136562 Slide into Action: Dynamic Shuttling of HIV Reverse Transcriptase on Nucleic Acid Substrates Science (New York, N.Y.). Nov, 2008 | Pubmed ID: 19008444 Structural Analysis of Protein-RNA Interactions with Mass Spectrometry Methods in Molecular Biology (Clifton, N.J.). 2008 | Pubmed ID: 18982294 Examining the Ribonuclease H Primer Grip of HIV-1 Reverse Transcriptase by Charge Neutralization of RNA/DNA Hybrids Nucleic Acids Research. Nov, 2008 | Pubmed ID: 18836193 Resistance to RevM10 Inhibition Reflects a Conformational Switch in the HIV-1 Rev Response Element Proceedings of the National Academy of Sciences of the United States of America. Sep, 2008 | Pubmed ID: 18776047 HIV-1 Ribonuclease H Inhibitory Phenolic Glycosides from Eugenia Hyemalis Journal of Natural Products. Sep, 2008 | Pubmed ID: 18763827 Mutations M184V and Y115F in HIV-1 Reverse Transcriptase Discriminate Against "nucleotide-competing Reverse Transcriptase Inhibitors" The Journal of Biological Chemistry. Oct, 2008 | Pubmed ID: 18728003 Dynamic Binding Orientations Direct Activity of HIV Reverse Transcriptase Nature. May, 2008 | Pubmed ID: 18464735 Structural Probing of the HIV-1 Polypurine Tract RNA:DNA Hybrid Using Classic Nucleic Acid Ligands Nucleic Acids Research. May, 2008 | Pubmed ID: 18400780 High-resolution NMR Analysis of the Conformations of Native and Base Analog Substituted Retroviral and LTR-retrotransposon PPT Primers Chemistry & Biology. Mar, 2008 | Pubmed ID: 18355725 An HIV RNase H Inhibitory 1,3,4,5-tetragalloylapiitol from the African Plant Hylodendron Gabunensis Journal of Natural Products. Oct, 2007 | Pubmed ID: 17935297 A Dimeric Lactone from Ardisia Japonica with Inhibitory Activity for HIV-1 and HIV-2 Ribonuclease H Journal of Natural Products. May, 2007 | Pubmed ID: 17397219 Investigating the "steric Gate" of Human Immunodeficiency Virus Type 1 (HIV-1) Reverse Transcriptase by Targeted Insertion of Unnatural Amino Acids Biochemistry. Feb, 2007 | Pubmed ID: 17274599 Exploiting Structurally Diverse Nucleoside Analogs As Probes of Reverse Transcription Complexes Current HIV Research. Jan, 2007 | Pubmed ID: 17266554 Purine Analog Substitution of the HIV-1 Polypurine Tract Primer Defines Regions Controlling Initiation of Plus-strand DNA Synthesis Nucleic Acids Research. 2007 | Pubmed ID: 17164285 Examining Interactions of HIV-1 Reverse Transcriptase with Single-stranded Template Nucleotides by Nucleoside Analog Interference The Journal of Biological Chemistry. Sep, 2006 | Pubmed ID: 16867979 Analysis of HIV-1 Replication Block Due to Substitutions at F61 Residue of Reverse Transcriptase Reveals Additional Defects Involving the RNase H Function Nucleic Acids Research. 2006 | Pubmed ID: 16723431 Nucleotide Excision Repair and Template-independent Addition by HIV-1 Reverse Transcriptase in the Presence of Nucleocapsid Protein The Journal of Biological Chemistry. Apr, 2006 | Pubmed ID: 16500895 Examining Ty3 Polypurine Tract Structure and Function by Nucleoside Analog Interference The Journal of Biological Chemistry. Feb, 2006 | Pubmed ID: 16306041 A Ribose Sugar Conformational Switch in the LTR-retrotransposon Ty3 Polypurine Tract-containing RNA/DNA Hybrid Journal of the American Chemical Society. Nov, 2005 | Pubmed ID: 16305191 Interaction of the Ty3 Reverse Transcriptase Thumb Subdomain with Template-primer The Journal of Biological Chemistry. Aug, 2005 | Pubmed ID: 15944162 Attenuation of DNA Replication by HIV-1 Reverse Transcriptase Near the Central Termination Sequence Biochemistry. Apr, 2005 | Pubmed ID: 15807528 Investigating HIV-1 Polypurine Tract Geometry Via Targeted Insertion of Abasic Lesions in the (-)-DNA Template and (+)-RNA Primer The Journal of Biological Chemistry. May, 2005 | Pubmed ID: 15778225 Selective Inhibition of HIV-1 Reverse Transcriptase-associated Ribonuclease H Activity by Hydroxylated Tropolones Nucleic Acids Research. 2005 | Pubmed ID: 15741178 Functional Roles of Carboxylate Residues Comprising the DNA Polymerase Active Site Triad of Ty3 Reverse Transcriptase Nucleic Acids Research. 2005 | Pubmed ID: 15647500 Mass Spectrometric Analysis of the HIV-1 Integrase-pyridoxal 5'-phosphate Complex Reveals a New Binding Site for a Nucleotide Inhibitor The Journal of Biological Chemistry. Mar, 2005 | Pubmed ID: 15615720 Site- and Subunit-specific Incorporation of Unnatural Amino Acids into HIV-1 Reverse Transcriptase Protein Expression and Purification. Nov, 2004 | Pubmed ID: 15477080 Synthetic TRNALys,3 As the Replication Primer for the HIV-1HXB2 and HIV-1Mal Genomes Nucleic Acids Research. 2004 | Pubmed ID: 15342789 A Capillary Electrophoretic Assay for Ribonuclease H Activity Analytical Biochemistry. Aug, 2004 | Pubmed ID: 15265735 Two Modes of HIV-1 Polypurine Tract Cleavage Are Affected by Introducing Locked Nucleic Acid Analogs into the (-) DNA Template The Journal of Biological Chemistry. Aug, 2004 | Pubmed ID: 15220330 'Binding, Bending and Bonding': Polypurine Tract-primed Initiation of Plus-strand DNA Synthesis in Human Immunodeficiency Virus The International Journal of Biochemistry & Cell Biology. Sep, 2004 | Pubmed ID: 15183342 Identification of an Inhibitor-binding Site to HIV-1 Integrase with Affinity Acetylation and Mass Spectrometry Proceedings of the National Academy of Sciences of the United States of America. May, 2004 | Pubmed ID: 15118107 Using Pyrrolo-deoxycytosine to Probe RNA/DNA Hybrids Containing the Human Immunodeficiency Virus Type-1 3' Polypurine Tract Nucleic Acids Research. 2004 | Pubmed ID: 15004241 A Fluorescence-based High-throughput Screening Assay for Inhibitors of Human Immunodeficiency Virus-1 Reverse Transcriptase-associated Ribonuclease H Activity Analytical Biochemistry. Nov, 2003 | Pubmed ID: 14705777 "In the Beginning": Initiation of Minus Strand DNA Synthesis in Retroviruses and LTR-containing Retrotransposons Biochemistry. Dec, 2003 | Pubmed ID: 14661945 Hydrolysis of RNA/DNA Hybrids Containing Nonpolar Pyrimidine Isosteres Defines Regions Essential for HIV Type 1 Polypurine Tract Selection Proceedings of the National Academy of Sciences of the United States of America. Sep, 2003 | Pubmed ID: 12972638 Human Immunodeficiency Virus Type 2 Reverse Transcriptase Activity in Model Systems That Mimic Steps in Reverse Transcription Journal of Virology. Jul, 2003 | Pubmed ID: 12805462 Role of the Reverse Transcriptase, Nucleocapsid Protein, and Template Structure in the Two-step Transfer Mechanism in Retroviral Recombination The Journal of Biological Chemistry. Aug, 2003 | Pubmed ID: 12801926 Effects of TRNA 3 Lys Aminoacylation on the Initiation of HIV-1 Reverse Transcription Biochimie. May, 2003 | Pubmed ID: 12763311 Nonpolar Thymine Isosteres in the Ty3 Polypurine Tract DNA Template Modulate Processing and Provide a Model for Its Recognition by Ty3 Reverse Transcriptase The Journal of Biological Chemistry. Jul, 2003 | Pubmed ID: 12730227 Uncovering the Complexities of Retroviral Ribonuclease H Reveals Its Potential As a Therapeutic Target AIDS Reviews. Oct-Dec, 2002 | Pubmed ID: 12555693 Identification of Specific HIV-1 Reverse Transcriptase Contacts to the Viral RNA:tRNA Complex by Mass Spectrometry and a Primary Amine Selective Reagent Proceedings of the National Academy of Sciences of the United States of America. Dec, 2002 | Pubmed ID: 12461175 Mutations in the Ribonuclease H Active Site of HIV-RT Reveal a Role for This Site in Stabilizing Enzyme-primer-template Binding Biochemistry. Sep, 2002 | Pubmed ID: 12206668 Direct and Indirect Contributions of RNA Secondary Structure Elements to the Initiation of HIV-1 Reverse Transcription The Journal of Biological Chemistry. Nov, 2002 | Pubmed ID: 12194974 Functional Interactions of Nucleocapsid Protein of Feline Immunodeficiency Virus and Cellular Prion Protein with the Viral RNA Journal of Molecular Biology. Apr, 2002 | Pubmed ID: 12054775 Mutating Conserved Residues in the Ribonuclease H Domain of Ty3 Reverse Transcriptase Affects Specialized Cleavage Events The Journal of Biological Chemistry. Jul, 2002 | Pubmed ID: 11994277 Altering the RNase H Primer Grip of Human Immunodeficiency Virus Reverse Transcriptase Modifies Cleavage Specificity Biochemistry. Apr, 2002 | Pubmed ID: 11939780 Pre-existing Distortions in Nucleic Acid Structure Aid Polypurine Tract Selection by HIV-1 Reverse Transcriptase The Journal of Biological Chemistry. May, 2002 | Pubmed ID: 11875059 Structural and Functional Properties of the HIV-1 RNA-tRNA(Lys)3 Primer Complex Annealed by the Nucleocapsid Protein: Comparison with the Heat-annealed Complex RNA (New York, N.Y.). Jan, 2002 | Pubmed ID: 11873759 Ty3 Integrase is Required for Initiation of Reverse Transcription Journal of Virology. Mar, 2002 | Pubmed ID: 11861848 РНК предсказание вторичной Структура Использование высоких-пропускная способность SHAPE Sabrina Lusvarghi*1, Joanna Sztuba-Solinska*1, Katarzyna J. Purzycka1, Jason W. Rausch1, Stuart F.J. Le Grice1 1RT Biochemistry Section, HIV Drug Resistance Program, Frederick National Laboratory for Cancer Research JoVE 50243 Biology
РНК предсказание вторичной Структура Использование высоких-пропускная способность SHAPE Sabrina Lusvarghi*1, Joanna Sztuba-Solinska*1, Katarzyna J. Purzycka1, Jason W. Rausch1, Stuart F.J. Le Grice1 1RT Biochemistry Section, HIV Drug Resistance Program, Frederick National Laboratory for Cancer Research JoVE 50243 Biology