Collecting Neural Stem and Progenitor Cells from Live Animals Using a Novel Brain Milking Protocol (Video) | JoVE

Dimitrios Dimitrakopoulos; Chrisitna Dimitriou; Freyja McClenahan; Robin J. M. Franklin; Ilias Kazanis

doi:10.3791/65308

Rat "Brain Milking" for Neural Stem and Oligodendrocyte Progenitor Cell Isolation

February 9th, 2024

Collecting Neural Stem and Progenitor Cells from Live Animals Using a Novel Brain Milking Protocol (Video) | JoVE

01:42 min

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ï»¿Releasing Murine Neural Stem Cells by Stereotaxic Intracerebroventricular Injection of Release Cocktail (Video) | JoVE

02:25 min

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Collecting Murine Neural Stem and Oligodendrocyte Progenitor Cells by Cerebrospinal Fluid Liquid Biopsy

02:52 min

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Dimitrios Dimitrakopoulos¹, Chrisitna Dimitriou¹, Freyja McClenahan², Robin J. M. Franklin^2,3, Ilias Kazanis¹

¹Laboratory of Developmental Biology, Department of Biology, University of Patras, ²Wellcome Trust-MRC Cambridge Stem Cell Institute, University of Cambridge, ³Altos Labs, Cambridge Institute of Science

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A method for the isolation of neural stem cells and oligodendrocyte progenitor cells from the brains of live rats is presented here in experimental detail. It allows multiple collections of these cells from the same animals without compromising their well-being.

Article

Rat "Brain Milking" for Neural Stem and Oligodendrocyte Progenitor Cell Isolation

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