China Academy of Engineering Physics 1 article published in JoVE Chemistry Preparation of Chitosan-based Injectable Hydrogels and Its Application in 3D Cell Culture Yongsan Li1, Yaling Zhang2, Yen Wei1, Lei Tao1 1The Key Laboratory of Bioorganic Phosphorus Chemistry & Chemical Biology (Ministry of Education), Department of Chemistry, Tsinghua University, 2Institute of Chemical Materials, China Academy of Engineering Physics Here we describe a facile preparation of chitosan-based injectable hydrogels using dynamic imine chemistry. Methods to adjust the hydrogel’s mechanical strength and its application in 3D cell culture are presented.
