The Academy of Integrative Medicine of Fudan University 1 article published in JoVE Medicine Preparation of Herbal Medicine: Er-Xian Decoction and Er-Xian-containing Serum for In Vivo and In Vitro Experiments Shufen Liu1, Yueli Sun1, Ji Li1, Jingcheng Dong2, Qin Bian1,2 1Longhua Hospital, Shanghai University of Traditional Chinese Medicine, 2The Academy of Integrative Medicine of Fudan University Here, we present the preparation of an er-xian decoction (EXD) in four steps—soaking, decoction, filtration, and concentration—and demonstrate the administration of a prepared EXD-containing serum to rats. These methods are applicable to the in vivo and in vitro study of herbal decoctions such as traditional Chinese medicines.