The First Affiliated Hospital of Bengbu Medical College 3 articles published in JoVE Medicine Establishing a Device for Sleep Deprivation in Mice Jianqing Chen*1, Jingze Wei*1, Xiaoying Ying*2, Fan Yang*2, Yichao Zhao*2, Jun Pu1,2 1Department of Cardiology, The First Affiliated Hospital of Bengbu Medical College, 2Department of Cardiology, Ren Ji Hospital, School of Medicine, Shanghai Jiao Tong University The present protocol outlines a method for setting up a cost-effective rocker platform-based device used for inducing sleep deprivation in mice. This device has proven to be effective in causing disruptions in electroencephalogram (EEG)-evidenced sleep patterns, as well as inducing metabolic and molecular changes associated with sleep deprivation. Medicine A Modified Simple Method for Induction of Myocardial Infarction in Mice Chenglin Jiang*1,2, Jianqing Chen*1,2, Yichao Zhao*2, Dasheng Gao1, Hongju Wang1, Jun Pu1,2 1Department of Cardiology, The First Affiliated Hospital of Bengbu Medical College, 2Department of Cardiology, Ren Ji Hospital, School of Medicine, Shanghai Jiao Tong University Under adequate anesthesia, the mouse heart was externalized through the intercostal space, and myocardial infarction was successfully induced by ligating the left anterior descending artery (LAD) using materials readily available in most laboratories. Biology A Simple and Efficient Method for In Vivo Cardiac-specific Gene Manipulation by Intramyocardial Injection in Mice Yanan Fu*1,2, Wenlong Jiang*2, Yichao Zhao*2, Yuli Huang1, Heng Zhang1, Hongju Wang1, Jun Pu1,2 1Department of Cardiology, The First Affiliated Hospital of Bengbu Medical College, 2Department of Cardiology, Ren Ji Hospital, School of Medicine, Shanghai Jiao Tong University Here we present a protocol for cardiac-specific gene manipulation in mice. Under anesthesia, the mouse hearts were externalized through the fourth intercostal space. Subsequently, adenoviruses encoding specific genes were injected with a syringe into the myocardium, followed by protein expression measurement via in vivo imaging and Western blot analysis.
