Jiaxing Tongji Institute of Environment 1 article published in JoVE Environment Using Caenorhabditis elegans for Studying Trans- and Multi-Generational Effects of Toxicants Zhuo Li1,2, Fangting Ai3, Jing Zhang4, Zhenyang Yu1,2, Daqiang Yin1,2 1College of Environmental Sciences and Engineering, Tongji University, 2Shanghai Institute of Pollution Control and Ecological Security, 3Jiaxing Tongji Institute of Environment, 4College of Ecological Technique and Engineering, Shanghai Institute of Technology Trans- and multi-generational effects of persistent chemicals are essential in judging their long-term consequences in the environment and on the human health. We provide novel detailed methods for studying trans- and multi-generational effects using free-living nematode Caenorhabditis elegans.