Sun Yat Sen Memorial Hospital 1 article published in JoVE Immunology and Infection Modeling Hepatitis B Virus Infection in Non-Hepatic 293T-NE-3NRs Cells Xiaoyue Sun*1,2,3, Xiaoqiang Yang*1,2,3,4, Chui Zeng1,2,3, Fayed Attia Koutb Megahed1,2,3,5, Qi Zhou1,2,3, Tingdang Liu1,2,3, Waqas Iqbal1,2,3, Pingnan Sun1,2,3, Xiaoling Zhou1,2,3 1Stem Cell Research Center, Shantou University Medical College, 2The Center for Reproductive Medicine, Shantou University Medical College, 3Guangdong Provincial Key Laboratory of Infectious Diseases and Molecular Immunopathology, Shantou University Medical College, 4Medical Research Center, Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University, 5Department of Nucleic Acid Researches, Genetic Engineering and Biotechnology Research Institute, General Autority - City of Scientific Researches and Technological Applications This manuscript describes a detailed protocol for Hepatitis B virus (HBV) infection in novel engineered 293T cells (293T-NE-3NRs, expressing human NTCP, HNF4α, RXRα and PPARα) and traditional hepatic cells (HepG2-NE, expressing human NTCP).
