University of the Chinese Academy of Sciences 1 article published in JoVE Biology Two-layered Membrane Sandwich Method for Laodelphax striatellus Saliva Collection Jing Zhao1,2,3, Jie Yang1,2,3, Lili Zhang1,2, Rongxiang Fang1,2, Yan Huo1,2 1State Key Laboratory of Plant Genomics, Institute of Microbiology, Chinese Academy of Sciences, 2National Plant Gene Research Center, 3University of the Chinese Academy of Sciences The present protocol describes a method to collect sufficient saliva from piercing-sucking insects using an artificial medium. This is a convenient method for collecting insect saliva and studying salivary function on insect feeding behavior and vector-borne virus transmission.
