Christian Medical College Campus 1 article published in JoVE Biology CRISPR/Cas9 Gene Editing of Hematopoietic Stem and Progenitor Cells for Gene Therapy Applications Vigneshwaran Venkatesan*1,2, Abisha Crystal Christopher*1,3, Karthik V. Karuppusamy1,2, Prathibha Babu1,2, Manoj Kumar K. Alagiri1,2, Saravanabhavan Thangavel1 1Centre for Stem Cell Research (CSCR), A unit of InStem Bengaluru, Christian Medical College campus, 2Manipal Academy of Higher Education, 3Thiruvalluvar University The present protocol describes an optimized hematopoietic stem and progenitor cell (HSPC) culture procedure for the robust engraftment of gene-edited cells in vivo.
