DBT-National Brain Research Centre 1 article published in JoVE Neuroscience In vivo Assessment of Microtubule Dynamics and Orientation in Caenorhabditis elegans Neurons Swagata Dey1, Anindya Ghosh-Roy1 1DBT-National Brain Research Centre A protocol for imaging the dynamic microtubules in vivo using fluorescently labeled End binding protein has been presented. We described the methods to label, image, and analyze the dynamic microtubules in the Posterior lateral microtubule (PLM) neuron of C. elegans.