CSIR Central Institute of Medicinal and Aromatic Plants 1 article published in JoVE Biology A Simple Protocol for Mapping the Plant Root System Architecture Traits Devesh Shukla1,2, Prabodh Kumar Trivedi2,3, Shivendra Sahi1,4 1Department of Biology, Western Kentucky University, 2Plant Biotechnology Division, CSIR Central Institute of Medicinal and Aromatic Plants, 3Academy of Scientific and Innovative Research (AcSIR), 4 We use simple laboratory tools to examine the root system architecture (RSA) of Arabidopsis and Medicago. The plantlets are grown hydroponically over mesh and spread using an art brush to reveal the RSA. Images are taken using scanning or a high-resolution camera, then analyzed with ImageJ to map traits.
