ICAR-Research Complex for Eastern Region 1 article published in JoVE Immunology and Infection Enrichment of Native and Recombinant Extracellular Vesicles of Mycobacteria Praapti Jayaswal1, Mohd Ilyas1, Kuljit Singh1,2, Saurabh Kumar1,3, Lovely Sisodiya1, Sapna Jain1, Rahul Mahlawat1, Nishant Sharma1,4, Vishal Gupta1, Krishnamohan Atmakuri1 1Bacterial Pathogenesis Laboratory, Infectious Diseases and Immunology Group, Translational Health Science and Technology Institute, NCR Biotech Science Cluster, 2Clinical Microbiology Division, CSIR-Indian Institute of Integrative Medicine, 3ICAR-Research Complex for Eastern Region, 4Public Health Research Institute, Rutgers University This protocol details the enrichment of native mycobacterial extracellular vesicles (mEVs) from axenic cultures of Mycobacterium smegmatis (Msm) and how mCherry (a red fluorescent reporter)-containing recombinant MsmEVs can be designed and enriched. Lastly, it verifies the novel approach with the enrichment of MsmEVs containing the EsxA protein of Mycobacterium tuberculosis.
