Jawaharlal Nehru Centre for Advanced Scientific Research (JNCASR) 1 article published in JoVE Biology Single-Cell Sorting of Immunophenotyped Mesenchymal Stem Cells from Human Exfoliated Deciduous Teeth Ayona Gupta*1, Risani Mukhopadhyay*1, Himanshi Khandelwal1, Narendra Nala2, Uttara Chakraborty1 1Manipal Institute of Regenerative Medicine, Bengaluru, Manipal Academy of Higher Education, Manipal, 2HIV-AIDS Laboratory, Molecular Biology and Genetics Unit, Jawaharlal Nehru Centre for Advanced Scientific Research (JNCASR) This protocol describes the use of fluorescence-activated cell sorting of human mesenchymal stem cells using the single-cell sorting method. Specifically, the use of single-cell sorting can achieve 99% purity of the immunophenotyped cells from a heterogeneous population when combined with a multiparametric flow cytometry-based approach.
