Anjo Kosei Hospital 1 article published in JoVE Biology Use of Anti-phospho-girdin Antibodies to Visualize Intestinal Tuft Cells in Free-Floating Mouse Jejunum Cryosections Yuka Mizutani1, Daisuke Kuga2, Machiko Iida1, Kaori Ushida3, Tsuyoshi Takagi1, Yoshihito Tokita1, Masahide Takahashi3, Masato Asai1,3 1Division of Perinatology, Institute for Developmental Research, Aichi Human Service Center, 2Surgery Department, Anjo Kosei Hospital, 3Department of Pathology, Nagoya University Graduate School of Medicine Kuga et al. discovered that phosphorylation-status specific antibodies against the actin binding protein girdin phosphorylated at tyrosine 1798 (pY1798) can be used to label tuft cells (TCs). This protocol allows robust visualization of TCs using immunofluorescent staining of free-floating jejunum cryosections with pY1798 antibodies.
