National Institute for Basic Biology 2 articles published in JoVE Developmental Biology Injecting Gryllus bimaculatus Eggs Samantha K. Barry1, Taro Nakamura2, Yuji Matsuoka3, Christoph Straub4, Hadley W. Horch4, Cassandra G. Extavour5 1Colby College, 2Division of Evolutionary Developmental Biology, National Institute for Basic Biology, 3Department of Biological Sciences, National University of Singapore, 4Department Biology and Department of Neuroscience, Bowdoin College, 5Department of Organismic and Evolutionary Biology and Department of Molecular and Cellular Biology, Harvard University Here we present a protocol to inject cricket eggs, a technique which serves as a foundational method in many experiments in the cricket, including, but not limited to, RNA interference and genomic manipulation. Biochemistry An Efficient Method for the Isolation of Highly Purified RNA from Seeds for Use in Quantitative Transcriptome Analysis Masatake Kanai1, Shoji Mano1,2, Mikio Nishimura3 1Laboratory of Biological Diversity, Department of Evolutionary Biology and Biodiversity, National Institute for Basic Biology, 2Department of Basic Biology, SOKENDAI (The Graduate University for Advanced Studies), 3Department of Cell Biology, National Institute for Basic Biology We have succeeded in establishing a method for RNA isolation from plant seeds containing large amounts of oils, proteins, and polyphenols, which have inhibitory effects on high-purity RNA isolation. Our method is suitable for monitoring the expression of genes with low level transcripts in seeds.
