Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences 1 article published in JoVE Environment Detection of 3-Nitrotyrosine in Atmospheric Environments via a High-performance Liquid Chromatography-electrochemical Detector System Tatsuo Ito1, Keiki Ogino1, Kenjiro Nagaoka1, Kei Takemoto1, Rina Nishiyama1, Yurika Shimizu2 1Department of Public Health, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences, 2Department of Pathophysiology-Periodontal Science, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences We present a method to detect 3-nitrotyrosine chemical modifications of atmospheric proteins with 6 mm-diameter rounds cut from air sampler prefilters using a high-performance liquid chromatography-electrochemical detector (HPLC-ECD).