Otsuka Pharmaceutical Co., Ltd. 1 article published in JoVE Medicine Lung Fixation under Constant Pressure for Evaluation of Emphysema in Mice Keiko Karasutani1,2, Hario Baskoro1, Tadashi Sato1, Naoko Arano1, Yohei Suzuki1, Aki Mitsui1, Naoko Shimada1, Yuzo Kodama1, Kuniaki Seyama1, Yoshinosuke Fukuchi1, Kazuhisa Takahashi1 1Department of Respiratory Medicine, Juntendo University Graduate School of Medicine, Juntendo University, 2Pharmaceutical Planning Group, Otsuka Pharmaceutical Co., Ltd. Presented here is a useful protocol for lung fixation that creates a stable condition for histological evaluate of lung specimens from a mouse model of emphysema. The main advantage of this model is that it can fix many lungs with the same constant pressure without lung collapse or deflation.