Jiksak Bioengineering, Inc. 1 article published in JoVE Neuroscience Three-Dimensional Motor Nerve Organoid Generation Tatsuya Osaki1,2, Siu Yu A. Chow1,2, Yui Nakanishi1,2, Joel Hernández1,3, Jiro Kawada4, Teruo Fujii1, Yoshiho Ikeuchi1,2 1Institute of Industrial Science, The University of Tokyo, 2Department of Chemistry and Biotechnology, School of Engineering, The University of Tokyo, 3Faculty of Science and Engineering, Tecnologico de Monterrey, 4Jiksak Bioengineering, Inc. This protocol provides a comprehensive procedure to fabricate human iPS cell-derived motor nerve organoid through spontaneous assembly of a robust bundle of axons extended from a spheroid in a tissue culture chip.