Korea Food Research Institute 1 article published in JoVE Biology Quantification, Viability Assessment, and Visualization Strategies for Acinetobacter Biofilms Joo-Sung Kim1,2, Jihoon Lim3 1Korea Food Research Institute, 2Department of Food Biotechnology, Korea University of Science and Technology, 3Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute This protocol describes the preparation of the inoculum, the biofilm quantification on microtiter plates using crystal violet dye, the viable count in biofilms, and the visualization of biofilms of Acinetobacter.