Universiti Malaya 4 articles published in JoVE Biology Monitoring Changes in Human Umbilical Vein Endothelial Cells upon Viral Infection Using Impedance-Based Real-Time Cell Analysis Jo-Ern Wong1, Nurhafiza Zainal2, Sazaly AbuBakar1, Kim-Kee Tan1 1Tropical Infectious Diseases Research and Education Centre (TIDREC), Higher Institution Center of Excellence (HICoE), Universiti Malaya, 2Department of Medical Microbiology, Faculty of Medicine, Universiti Malaya The current study describes a protocol to monitor the changes in human umbilical vein endothelial cells (HUVECs) during viral infection using a real-time cell analysis (RTCA) system. Immunology and Infection Virus Propagation and Cell-Based Colorimetric Quantification Jia-Yi Tan1, Jo-Ern Wong1, Nurhafiza Zainal2, Sazaly AbuBakar1, Kim-Kee Tan1 1Tropical Infectious Diseases Research and Education Centre (TIDREC), Higher Institution Center of Excellence (HICoE), Universiti Malaya, 2Department of Medical Microbiology, Faculty of Medicine, Universiti Malaya The present protocol describes the propagation of Zika virus (ZIKV) in Vero African green monkey kidney cells and the quantification of ZIKV using cell-based colorimetric immunodetection methods in 24-well and 96-well (high throughput) formats. Biology In Silico Identification and Characterization of circRNAs During Host-Pathogen Interactions Mathanakumara Ealam Selvan*1, Kai Shen Lim*1, Chee How Teo2, Yat-Yuen Lim1 1Institute of Biological Sciences, Faculty of Science, Universiti Malaya, 2Centre for Research in Biotechnology for Agriculture (CEBAR), Universiti Malaya The protocol submitted here explains the complete in silico pipeline needed to predict and functionally characterize circRNAs from RNA-sequencing transcriptome data studying host-pathogen interactions. Neuroscience Locomotor Assessment of 6-Hydroxydopamine-induced Adult Zebrafish-based Parkinson's Disease Model Naemah Md Hamzah1, Siong Meng Lim1, Yuganthini Vijayanathan1,2, Fei Tieng Lim1, Abu Bakar Abdul Majeed1, Maw Pin Tan2, Kalavathy Ramasamy1 1Collaborative Drug Discovery Research (CDDR) Group and Brain Degeneration and Therapeutics Group, Faculty of Pharmacy, Universiti Teknologi MARA (UiTM), Cawangan Selangor, Kampus Puncak Alam, 2Department of Medicine, Faculty of Medicine, Universiti Malaya The present protocol describes the intracerebroventricular (ICV) injection of adult zebrafish with neurotoxic 6-hydroxydopamine (6-OHDA) at the ventral diencephalon (Dn) and the assessment of the impairment and subsequent recovery of swimming behavior postlesion by using the open tank test, which is accompanied by analysis using a video tracking software.
