Skolkovo Innovation Center 1 article published in JoVE Biochemistry Imaging of Extracellular Vesicles by Atomic Force Microscopy Mikhail Skliar1,2, Vasiliy S. Chernyshev3,4 1Department of Chemical Engineering, University of Utah, 2The Nano Institute of Utah, University of Utah, 3Center for Photonics and Quantum Materials, Skolkovo Institute of Science and Technology, 4Biopharmaceutical Cluster 'Northern', Moscow Institute of Physics and Technology A step-by-step procedure is described for label-free immobilization of exosomes and extracellular vesicles from liquid samples and their imaging by atomic force microscopy (AFM). The AFM images are used to estimate the size of the vesicles in the solution and characterize other biophysical properties.
