Roche Innovation Center Basel 2 articles published in JoVE Neuroscience Methods for the Discovery of Novel Compounds Modulating a Gamma-Aminobutyric Acid Receptor Type A Neurotransmission Frédéric Knoflach1, Maria-Clemencia Hernandez1, Daniel Bertrand2 1Discovery Neuroscience, Pharma Research and Early Development, Roche Innovation Center Basel, 2HiQScreen Sàrl 6 Here, we present protocols to discover compounds active at GABAA receptors, from the binding to the physiology and pharmacology. Neuroscience High-resolution Confocal Imaging of the Blood-brain Barrier: Imaging, 3D Reconstruction, and Quantification of Transcytosis Roberto Villaseñor1, Ludovic Collin1 1Roche Pharma Research and Early Development (pRED), Neuroimmunology, Roche Innovation Center Basel Here we present a microscopy-based protocol for high-resolution imaging and a three-dimensional reconstruction of the mouse neurovascular unit and blood-brain barrier using brain free-floating sections. This method allows for the visualization, analysis, and quantification of intracellular organelles at the BBB.
