Roche Innovation Centre Basel 1 article published in JoVE Biology A Simple, Quick, and Partially Automated Protocol for the Isolation of Single Nuclei from Frozen Mammalian Tissues for Single Nucleus Sequencing Lucas Stalder1, Fabian Koechl1, Kerstin Hahn1, Marc Sultan1, Megana K. Prasad1 1Roche Pharma Research and Early Development, Pharmaceutical Sciences, Roche Innovation Centre Basel The study describes a simple, quick, and partially automated protocol to isolate high-quality nuclei from frozen mammalian tissues for downstream single nuclei RNA sequencing.
