Research Institute of Biopharmacy and Veterinary Drugs 1 article published in JoVE Immunology and Infection Loop-mediated Isothermal Amplification (LAMP) Assays for the Species-specific Detection of Eimeria that Infect Chickens Christopher P. Barkway1, Rebecca L. Pocock1, Vladimir Vrba2, Damer P. Blake1 1Department of Pathology and Pathogen Biology, Royal Veterinary College, London, 2BIOPHARM, Research Institute of Biopharmacy and Veterinary Drugs Diagnosis of Eimeria infection in chickens remains demanding. Parasite morphology- and host pathology-led approaches are commonly inconclusive, while molecular approaches based on PCR have proven demanding in cost and expertise. The aim of this protocol is to establish loop-mediated isothermal amplification (LAMP) as a straightforward molecular diagnostic for eimerian infection.
