Czech-BioImaging IPHYS, Institute of Physiology of the Czech Academy of Sciences 1 article published in JoVE Biology Measurement of Liver Stiffness Using Atomic Force Microscopy Coupled with Polarization Microscopy Srikant Ojha1, Jan Pribyl2, Simon Klimovic2,3, Daniel Hadraba4, Marketa Jirouskova1, Martin Gregor1 1Laboratory of Integrative Biology, Institute of Molecular Genetics of the Czech Academy of Sciences, 2CEITEC MU, Masaryk University, 3Department of Biochemistry, Faculty of Science, Masaryk University, 4Laboratory of Biomathematics, Czech-BioImaging IPHYS, Institute of Physiology of the Czech Academy of Sciences We present a protocol to measure the elastic moduli of collagen-rich areas in normal and diseased liver using atomic force microscopy. The simultaneous use of polarization microscopy provides high spatial precision for localizing collagen-rich areas in the liver sections.
