Munich Cluster for Systems Neurology (SyNergy) 5 articles published in JoVE Neuroscience Microfluidics-Assisted Selective Depolarization of Axonal Mitochondria Simone Wanderoy*1,2, Alina Rühmkorf*1,2, Angelika B. Harbauer2,3,4 1TUM Medical Graduate Center, Technical University of Munich, 2Max Planck Institute of Neurobiology, 3TUM School of Medicine, Institute of Neuronal Cell Biology, Technical University of Munich, 4Munich Cluster for Systems Neurology The present protocol describes the seeding and staining of neuronal mitochondria in microfluidic chambers. The fluidic pressure gradient in these chambers allows for the selective treatment of mitochondria in axons to analyze their properties in response to pharmacological challenges without affecting the cell body compartment. Neuroscience Modeling Stroke in Mice: Transient Middle Cerebral Artery Occlusion via the External Carotid Artery Gemma Llovera1, Alba Simats1, Arthur Liesz1,2 1Institute for Stroke and Dementia Research, LMU Munich, 2Munich Cluster for Systems Neurology (SyNergy) Different models of middle cerebral artery occlusion (MCAo) are used in experimental stroke research. Here, an experimental stroke model of transient MCAo via the external carotid artery (ECA) is described, which aims to mimic human stroke, in which the cerebrovascular thrombus is removed due to spontaneous clot lysis or therapy. Neuroscience Modeling Stroke in Mice: Focal Cortical Lesions by Photothrombosis Gemma Llovera1, Kelsey Pinkham1, Arthur Liesz1,2 1Institute for Stroke and Dementia Research, LMU Munich, 2Munich Cluster for Systems Neurology (SyNergy) Described here is the photothrombotic stroke model, where a stroke is produced through the intact skull by inducing permanent microvascular occlusion using laser illumination after administration of a photosensitive dye. Developmental Biology Electroporation Method for In Vivo Delivery of Plasmid DNA in the Adult Zebrafish Telencephalon Tamara Durovic1,2, Jovica Ninkovic1,3,4 1Institute of Stem Cell Research, Helmholtz Zentrum München, 2Graduate School of Systemic Neurosciences, Ludwig Maximilian University, 3Department of Cell Biology and Anatomy, Biomedical Center, Ludwig Maximilian University, 4Munich Cluster for Systems Neurology (SyNergy) Presented here is an electroporation method for plasmid DNA delivery and ependymoglial cell labeling in the adult zebrafish telencephalon. This protocol is a quick and efficient method to visualize and trace individual ependymoglial cells and opens up new possibilities to apply electroporation to a broad range of genetic manipulations. Medicine Modeling Stroke in Mice: Permanent Coagulation of the Distal Middle Cerebral Artery Gemma Llovera*1,2, Stefan Roth*1,2, Nikolaus Plesnila1,2, Roland Veltkamp3,4, Arthur Liesz1,2 1Institute for Stroke and Dementia Research, University Hospital Munich, 2Munich Cluster for Systems Neurology (SyNergy), 3Department of Neurology, University Heidelberg, 4Imperial College, Charing Cross Hospital Various murine models of middle cerebral artery occlusion (MCAO) are widely used in experimental brain research. Here, we demonstrate the model of transcranial permanent distal MCAO which produces consistent cortical infarction of a size corresponding to damage imposed by the majority of human ischemic strokes.
