Federal Institute for Geosciences and Natural Resources 1 article published in JoVE Engineering Using Flexible Gold-Titanium Reaction Cells to Simulate Pressure-Dependent Microbial Activity in the Context of Subsurface Biomining Christian Ostertag-Henning1, Ruiyong Zhang1, Oliver Helten1, Thomas Weger1, Axel Schippers1 1Federal Institute for Geosciences and Natural Resources This protocol describes microbial experiments under elevated pressures to study in situ biomining processes. The experimental approach employs a rocking high-pressure reactor equipped with a gold-titanium reaction cell containing a microbial culture in an acidic, iron-rich medium.