Centro de Investigacion Biomedica en Red de Diabetes y Enfermedades Metabolicas Asociadas (CIBERDEM) 1 article published in JoVE Medicine A High-Throughput In Situ Method for Estimation of Hepatocyte Nuclear Ploidy in Mice Fátima Manzano-Núñez1, Ruby Peters2, Deborah J. Burks1,3, Luke A. Noon1,3 1Centro de Investigación Príncipe Felipe (CIPF), 2Department of Physiology, Development and Neuroscience, University of Cambridge, 3Centro de Investigación Biomédica en Red de Diabetes y Enfermedades Metabólicas Asociadas (CIBERDEM) We present a robust, cost-effective, and flexible method for measuring changes in hepatocyte number and nuclear ploidy within fixed/cryopreserved tissue samples that does not require flow cytometry. Our approach provides a powerful sample-wide signature of liver cytology ideal for tracking the progression of liver injury and disease.
