Flow Cytometry and Cell Sorting Platform (ISPA) 1 article published in JoVE Biology Immunophenotyping and Cell Sorting of Human MKs from Human Primary Sources or Differentiated In Vitro from Hematopoietic Progenitors Andrea Acebes-Huerta1, Patricia Martínez-Botía1, Cristina Martín Martín2, Ángel Bernardo1,3, Adrián R. Rodríguez1, María Carmen Vicente-Ayuso4, Celina Benavente Cuesta5, Laura Gutiérrez1,6 1Platelet Research Lab, Instituto de Investigación Sanitaria del Principado de Asturias (ISPA), 2Flow Cytometry and Cell Sorting Platform (ISPA), 3Clinical Diagnosis Laboratory - Dept. of Hematology, Hospital Universitario Central de Asturias (HUCA), 4Department of Hematology, Hospital Universitario Severo Ochoa (HUSO), 5Department of Hematology, Instituto de Investigación Sanitaria San Carlos (IdISSC), Hospital Clínico San Carlos (HCSC), 6Dept. of Medicine, University of Oviedo Here, we present an immunophenotyping strategy for the characterization of megakaryocyte differentiation, and show how that strategy allows the sorting of megakaryocytes at different stages with a fluorescence-activated cell sorter. The methodology can be applied to human primary tissues, but also to megakaryocytes generated in culture in vitro.
