INRA Universite de Lorraine 3 articles published in JoVE Bioengineering Substructure Analyzer: A User-Friendly Workflow for Rapid Exploration and Accurate Analysis of Cellular Bodies in Fluorescence Microscopy Images Géraud Heckler1, Christelle Aigueperse1, Liza Hettal1, Quentin Thuillier1, Fabrice de Chaumont2, Stéphane Dallongeville2, Isabelle Behm-Ansmant1 1Université de Lorraine, CNRS, IMoPA F54000 Nancy France, 2Institut Pasteur, BioImage Analysis Unit, CNRS UMR 3691, Paris, France. We present a freely available workflow built for rapid exploration and accurate analysis of cellular bodies in specific cell compartments in fluorescence microscopy images. This user-friendly workflow is designed on the open-source software Icy and also uses ImageJ functionalities. The pipeline is affordable without knowledge in image analysis. Immunology and Infection A New Method for Qualitative Multi-scale Analysis of Bacterial Biofilms on Filamentous Fungal Colonies Using Confocal and Electron Microscopy Cora Miquel Guennoc1, Christophe Rose2, Frédéric Guinnet1, Igor Miquel1, Jessy Labbé3, Aurélie Deveau1 1Interactions Arbres – Microorganismes, UMR1136, INRA Université de Lorraine, 2Ecologie et Ecophysiologie Forestières - PTEF, UMR 1137, INRA Université de Lorraine, 3Biosciences Division, Oak Ridge National Laboratory This protocol describes a new method to grow and qualitatively analyze bacterial biofilms on fungal hyphae by confocal and electron microscopy. Biology Isolation of Viable Multicellular Glands from Tissue of the Carnivorous Plant, Nepenthes Sandy Rottloff1, Axel Mithöfer2, Ute Müller3, Roland Kilper3 1Laboratoire Agronomie et Environnement, Université de Lorraine, 2Department of Bioorganic Chemistry, Max Planck Institute for Chemical Ecology, 3aura optik Plants glands are specialized structures responsible for the biosynthesis and secretion of many compounds involved in interactions with the biotic environment. To enable studies on their molecular and biochemical features, a mechanical micropreparation technique was established in order to isolate single metabolically active glands, here from the carnivorous plant Nepenthes.
