Univ. Artois 1 article published in JoVE Biology A Triple Culture Cell System Modeling the Human Blood-Brain Barrier Eleonora Rizzi*1, Clémence Deligne*1, Lucie Dehouck1, Roberta Bilardo2, Yasutero Sano3, Fumitaka Shimizu3, Takashi Kanda3, Marina Resmini2, Fabien Gosselet1, Marie-Pierre Dehouck1, Caroline Mysiorek1 1Univ. Artois, UR 2465, Laboratoire de la Barrière Hémato-Encéphalique (LBHE), 2Queen Mary University of London, Department of Chemistry, 3Yamaguchi University Graduate School of Medicine, Department of Neurology and Clinical Neuroscience This protocol describes a method to establish a human blood-brain barrier (BBB) in vitro model. The endothelial cells and pericytes are seeded on each side of an insert filter (blood compartment), and astrocytes are seeded in the bottom well (brain compartment). The model characterized was used for nanoparticles transport experiments.
