BHF Centre of Research Excellence 1 article published in JoVE Developmental Biology Genotyping and Quantification of In Situ Hybridization Staining in Zebrafish Tomasz Dobrzycki*1,2, Monika Krecsmarik*1,2, Rui Monteiro1,2,3 1MRC Molecular Haematology Unit, MRC Weatherall Institute of Molecular Medicine, John Radcliffe Hospital, University of Oxford, 2BHF Centre of Research Excellence, 3Institute of Cancer and Genomic Sciences, University of Birmingham Gene editing technologies have enabled researchers to generate zebrafish mutants to investigate gene function with relative ease. Here, we provide a guide to perform parallel embryo genotyping and quantification of in situ hybridization signals in zebrafish. This unbiased approach provides greater accuracy in phenotypical analyses based on in situ hybridization.
