MSD R&D Innovation Centre 2 articles published in JoVE Neuroscience Three-Dimensional Bioprinting of Human iPSC-Derived Neuron-Astrocyte Cocultures for Screening Applications Chloe Ann Whitehouse1, Yufang He2, Janet Brownlees1, Nicola Corbett1 1MSD Research Laboratories, London, UK, 2Merck & Co., Inc., Rahway, NJ, USA Here, we present a protocol to produce 3D-bioprinted cocultures of iPSC-derived neurons and astrocytes. This coculture model, generated within a hydrogel scaffold in 96- or 384-well formats, demonstrates high post-print viability and neurite outgrowth within 7 days and shows the expression of maturity markers for both cell types. Neuroscience Fluorescence-Activated Nuclei Negative Sorting of Neurons Combined with Single Nuclei RNA Sequencing to Study the Hippocampal Neurogenic Niche Thomas Kerloch1, Tjaša Lepko2, Kirill Shkura2, François Guillemot1, Sébastien Gillotin2 1The Francis Crick Institute, 2MSD R&D Innovation Centre Presented here is a method to sequence single nuclei isolated from the mouse dentate gyrus that excludes most neurons through fluorescence-activated nuclei (FAN)-sorting. This approach generates high-quality expression profiles and facilitates the study of most other cell types represented in the niche, including scarce populations such as neural stem cells.
