Erasmus MC 11 articles published in JoVE Immunology and Infection A Multimodal Imaging Approach Based on Micro-CT and Fluorescence Molecular Tomography for Longitudinal Assessment of Bleomycin-Induced Lung Fibrosis in Mice Francesca Ruscitti1, Francesca Ravanetti2, Gaetano Donofrio2, Yanto Ridwan3, Paula van Heijningen3, Jeroen Essers4, Gino Villetti1, Antonio Cacchioli2, Wim Vos5, Franco Fabio Stellari1 1Corporate Preclinical R&D, Chiesi Farmaceutici S.p.A., 2Department of Veterinary Science, University of Parma, 3Department of Molecular Genetics, Erasmus MC, 4Department of Molecular Genetics, Vascular Surgery, Radiation Oncology, Erasmus MC, 5Fluidda NV We describe a non-invasive multimodal imaging approach based on Micro-CT and fluorescence molecular tomography for longitudinal assessment of the mouse lung fibrosis model induced by double intratracheal instillation of bleomycin. Medicine Intravital Microscopy of Tumor-associated Vasculature Using Advanced Dorsal Skinfold Window Chambers on Transgenic Fluorescent Mice Ann L.B. Seynhaeve1, Timo L.M. ten Hagen1 1Laboratory Experimental Surgical Oncology, Section Surgical Oncology, Department of Surgery, Erasmus MC This protocol describes the intravital imaging of transgenic mice expressing cell-specific fluorescent markers. Intravital imaging provides a non-invasive method for high-resolution observations in living animals using implantable windows through which the microscopic visualization of processes is possible. This method is especially useful to study longitudinal processes. Medicine Using In Vitro Live-cell Imaging to Explore Chemotherapeutics Delivered by Lipid-based Nanoparticles Ann L.B. Seynhaeve1, Timo L.M. ten Hagen1 1Laboratory Experimental Surgical Oncology, Section Surgical Oncology, Department of Surgery, Erasmus MC Live-cell imaging is a powerful tool to visualize dynamic processes. The examination of fixed cells provides only static pictures, which can lead to misinterpretation and confusion about the process. This work presents a method to study uptake, drug release, and intracellular localization of liposomal nanoparticles in living cells. Medicine Percutaneous Hepatic Perfusion (PHP) with Melphalan as a Treatment for Unresectable Metastases Confined to the Liver Eleonora M. de Leede1, Mark C. Burgmans2, Christian H. Martini3, Fred G. J. Tijl4, Arian R. van Erkel2, Jaap Vuyk3, Ellen Kapiteijn5, Cornelis Verhoef6, Cornelis J. H. van de Velde1, Alexander L. Vahrmeijer1 1Department of Surgery, Leiden University Medical Centre, 2Department of Radiology, Leiden University Medical Centre, 3Department of Anesthesiology, Leiden University Medical Centre, 4Department of Extracorporeal Circulation, Leiden University Medical Centre, 5Department of Medical Oncology, Leiden University Medical Centre, 6Department of Surgery, Erasmus MC Cancer Institute In this manuscript, we describe percutaneous isolated hepatic perfusion with simultaneous chemofiltration as treatment for unresectable liver metastases. This procedure is performed under general anaesthesia in the angiosuite by an experienced team, consisting of an interventional radiologist, a clinical perfusionist and anaesthesiologist. Behavior A Method to Quantify Visual Information Processing in Children Using Eye Tracking Marlou J.G. Kooiker1, Johan J.M. Pel1, Sanny P. van der Steen-Kant2, Johannes van der Steen1,2 1Department of Neuroscience, Erasmus MC, 2KEI, Royal Dutch Visio A method is described to quantify the quality of visual information processing based on reflexive eye movements in response to specific visual modalities. Reaction times and fixation output parameters are used to characterize visual performance in children with and without visual impairments from 6 months of age. Developmental Biology Large-scale Scanning Transmission Electron Microscopy (Nanotomy) of Healthy and Injured Zebrafish Brain Jeroen Kuipers1, Ruby D. Kalicharan1, Anouk H. G. Wolters1, Tjakko J. van Ham*2, Ben N.G. Giepmans*1 1Cell Biology, UMC Groningen, 2Clinical Genetics, Erasmus MC Rotterdam Large-scale 2D electron microscopy (EM), or nanotomy, is the tissue-wide application of nanoscale resolution EM. Here we describe a universal method for nanotomy applied to investigate the zebrafish larval brain in health and upon non-invasive brain injury. Bioengineering In Vivo Quantitative Assessment of Myocardial Structure, Function, Perfusion and Viability Using Cardiac Micro-computed Tomography Elza van Deel1,2, Yanto Ridwan1, J. Nicole van Vliet1, Sasha Belenkov3, Jeroen Essers1,4,5 1Department of Genetics, Erasmus MC, Rotterdam, 2Department of Experimental Cardiology, Erasmus MC, Rotterdam, 3PerkinElmer, Inc., 4Department of Vascular Surgery, Erasmus MC, Rotterdam, 5Department of Radiation Oncology, Erasmus MC, Rotterdam This method outlines the use of Quantum Micro-Computed Tomography (MicroCT) to assess cardiac morphology, function, perfusion, metabolism and viability with iodinated contrast agent in mice with experimentally-induced myocardial ischemia. The technique can be applied for non-destructive high-throughput longitudinal in vivo imaging of various animal models of human heart disease. Medicine Surgical Placement of Catheters for Long-term Cardiovascular Exercise Testing in Swine Daphne P M De Wijs-Meijler*1, Kelly Stam*2, Richard W B van Duin1, Annemarie Verzijl2, Irwin K Reiss3, Dirk J Duncker2, Daphne Merkus2 1Experimental Cardiology and Neonatology, Erasmus MC, 2Experimental Cardiology, Erasmus MC, 3Neonatology, Erasmus MC Here we present a protocol to assess cardiopulmonary function in awake swine, at rest and during graded treadmill exercise. Chronic instrumentation allows for repeated hemodynamic measurements uninfluenced by cardiodepressive anesthetic agents. Medicine Transcutaneous Microcirculatory Imaging in Preterm Neonates Hugo van Elteren1, Irwin K.M. Reiss1, Rogier C.J. de Jonge1 1Department of Pediatrics, Division of Neonatology, Erasmus MC-Sophia Children's Hospital Microcirculatory imaging (MI) is used to monitor peripheral perfusion in critically ill or preterm neonates. This manuscript and video demonstrates the optimal approach for obtaining high-quality images. Medicine State of the Art Cranial Ultrasound Imaging in Neonates Ginette M. Ecury-Goossen1, Fleur A. Camfferman2, Lara M. Leijser3,4, Paul Govaert1,5, Jeroen Dudink1,2 1Department of Pediatrics, Division of Neonatology, Erasmus MC-Sophia Children's Hospital, 2Department of Radiology, Erasmus MC-Sophia Children's Hospital, 3Department of Pediatrics, Division of Neonatology, UZ Brussel, 4Department of Pediatrics, Division of Neonatology, Leiden University Medical Center, 5Department of Pediatrics, Division of Neonatology, Isala Hospital, 6 Cranial ultrasound (CUS) is a valuable tool for brain imaging in critically ill neonates. This video shows a comprehensive approach for neonatal (Doppler) CUS for both clinical and research purposes, including a bedside demonstration of the technique. Biology Isolation of Blood-vessel-derived Multipotent Precursors from Human Skeletal Muscle William C.W. Chen1, Arman Saparov2,3, Mirko Corselli4, Mihaela Crisan5, Bo Zheng6, Bruno Péault7,8, Johnny Huard9 1Stem Cell Research Center, Department of Bioengineering and Orthopedic Surgery, University of Pittsburgh, 2Department of Orthopedic Surgery, University of Pittsburgh, 3Nazarbayev University Research and Innovation System, Nazarbayev University, 4Department of Orthopaedic Surgery, UCLA Orthopaedic Hospital and the Orthopaedic Hospital Research Center, University of California at Los Angeles, 5Department of Cell Biology, Erasmus MC Stem Cell Institute, 6OHSU Center for Regenerative Medicine, Oregon Health & Science University, 7 Blood vessels within human skeletal muscle harbor several multi-lineage precursor populations that are ideal for regenerative applications. This isolation method allows simultaneous purification of three multipotent precursor cell populations respectively from three structural layers of blood vessels: myogenic endothelial cells from intima, pericytes from media, and adventitial cells from adventitia.
