Optics 11 life 1 article published in JoVE Bioengineering Experimental and Data Analysis Workflow for Soft Matter Nanoindentation Giuseppe Ciccone1, Mariana Azevedo Gonzalez Oliva1, Nelda Antonovaite2, Ines Lüchtefeld3, Manuel Salmeron-Sanchez1, Massimo Vassalli1 1Centre for the Cellular Microenvironment, James Watt School of Engineering, University of Glasgow, 2Optics 11 life, 3Laboratory of Biosensors and Bioelectronics, ETH Zürich The protocol presents a complete workflow for soft material nanoindentation experiments, including hydrogels and cells. First, the experimental steps to acquire force spectroscopy data are detailed; then, the analysis of such data is detailed through a newly developed open-source Python software, which is free to download from GitHub.
