Medical University of Silesia 3 articles published in JoVE Medicine The Use of Mixed Reality in Custom-Made Revision Hip Arthroplasty: A First Case Report Paweł Łęgosz1, Krzysztof Starszak1,2, Maciej Stanuch3,4, Maciej Otworowski1, Łukasz Pulik1, Adriana Złahoda-Huzior3,4, Andrzej Skalski3,4 1Department of Orthopaedics and Traumatology of the Musculoskeletal System, Infant Jesus Teaching Hospital, Medical University of Warsaw, 2Department of Human Anatomy, Medical University of Silesia, 3Department of Measurement and Electronics, AGH University of Science and Technology, 4MedApp S.A. A complex revision hip arthroplasty was performed using a custom-made implant and mixed reality technology. According to the authors' knowledge, this is the first report of such procedure described in the literature. Immunology and Infection Visualization of SARS-CoV-2 using Immuno RNA-Fluorescence In Situ Hybridization Anna Kula-Pacurar1, Jakub Wadas2, Agnieszka Suder1, Artur Szczepanski1,2, Aleksandra Milewska1,2, Marek Ochman3,4, Tomasz Stacel3,4, Krzysztof Pyrc1 1Malopolska Centre of Biotechnology, Jagiellonian University, 2Microbiology Department Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, 3Department of Cardiac, Vascular and Endovascular Surgery and Transplantology, The Medical University of Silesia in Katowice, 4Silesian Centre for Heart Diseases Here, we describe a simple method that combines RNA fluorescence in situ hybridization (RNA-FISH) with immunofluorescence to visualize severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) RNA. This protocol may increase understanding of the molecular characteristics of SARS-CoV-2 RNA-host interactions at a single-cell level. Biochemistry Detection of Small GTPase Prenylation and GTP Binding Using Membrane Fractionation and GTPase-linked Immunosorbent Assay Javad Alizadeh1,2, Shahla Shojaei1,2,3, Simone da Silva Rosa1, Adel Rezaei Moghadam1,2, Amir A. Zeki4, Mohammad Hashemi5, Marek J. Los6,7,8, Joseph W. Gordon1,9, Saeid Ghavami1,2,10 1Department of Human Anatomy and Cell Science, Rady Faculty of Health Sciences, Max Rady College of Medicine, University of Manitoba, 2Biology of Breathing Theme, Children Hospital Research Institute of Manitoba, University of Manitoba, 3Department of Biochemistry, School of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences, 4Division of Pulmonary, Critical Care, and Sleep Medicine, Department of Internal Medicine, Center for Comparative Respiratory Biology and Medicine, 5Department of Clinical Biochemistry, School of Medicine, Zahedan University of Medical Sciences, 6Department of Molecular Biology, School of Pharmacy with the Division of Laboratory Medicine in Sosnowiec, Medical University of Silesia, 7Centre de biophysique moléculaire - UPR 4301, Centre national de la recherche scientifique (CNRS) CS80054, 8LinkoCare Life Sciences AB, 9College of Nursing and Children's Hospital Research Institute of Manitoba, Rady Faculty of Health Sciences, University of Manitoba, 10Health Policy Research Center, Institute of Health, Shiraz University of Medical Sciences Here we describe a protocol to investigate the prenylation and guanosine-5'-triphosphate (GTP)-loading of Rho GTPase. This protocol consists of two detailed methods, namely membrane fractionation and a GTPase-linked immunosorbent assay. The protocol can be used for measuring the prenylation and GTP loading of different other small GTPases.
