Polish-Japanese Academy of Information Technology 1 article published in JoVE Biochemistry RIBO-seq in Bacteria: a Sample Collection and Library Preparation Protocol for NGS Sequencing Natalia Kopik1,2, Olga Chrobak1,2, Przemyslaw Latoch1,3, Mariia Kovalenko1,2, Agata L. Starosta1,2 1Laboratory of Gene Expression, ECOTECH-Complex, Maria Curie-Sklodowska University, 2Department of Molecular Biology, Institute of Biological Sciences, Maria Curie-Sklodowska University, 3Faculty of Information Technology, Polish-Japanese Academy of Information Technology Here we describe the stages of sample collection and preparation for RIBO-seq in bacteria. Sequencing of the libraries prepared according to these guidelines results in sufficient data for comprehensive bioinformatic analysis. The protocol we present is simple, uses standard laboratory equipment and takes seven days from lysis to obtaining libraries.
