NOVA School of Science and Technology 1 article published in JoVE Developmental Biology Three and Four-Dimensional Visualization and Analysis Approaches to Study Vertebrate Axial Elongation and Segmentation André Dias1, Gabriel G. Martins1,2, Alexandre Lopes1,3, Moisés Mallo1 1Instituto Gulbenkian de Ciência, 2Faculdade de Ciências da Universidade de Lisboa, 3NOVA School of Science and Technology Here, we describe computational tools and methods that allow visualization and analysis of three and four-dimensional image data of mouse embryos in the context of axial elongation and segmentation, obtained by in toto optical projection tomography, and by live imaging and whole-mount immunofluorescence staining using multiphoton microscopy.
