Canadian Food Inspection Agency 1 article published in JoVE Immunology and Infection Real-time Quaking-induced Conversion Assay for Detection of CWD Prions in Fecal Material Yo Ching Cheng*1, Samia Hannaoui*1, Theodore Ralph John2, Sandor Dudas3, Stefanie Czub3, Sabine Gilch1 1Dept. of Ecosystem and Public Health, Calgary Prion Research Units, Faculty of Veterinary Medicine, University of Calgary, 2Dept. of Molecular Biology, University of Wyoming, 3Canadian Food Inspection Agency, Lethbridge Laboratories Here, we present a protocol to describe a simple, fast and efficient prion amplification technique, the real-time quaking-induced conversion (RT-QuIC) method.
