Centre de Recherche du CHU de Quebec-Universite Laval 1 article published in JoVE Immunology and Infection Immunofluorescence Staining Using IBA1 and TMEM119 for Microglial Density, Morphology and Peripheral Myeloid Cell Infiltration Analysis in Mouse Brain Fernando González Ibanez1,2, Katherine Picard1,2, Maude Bordeleau1,3, Kaushik Sharma1,2,4, Kanchan Bisht1,2,4, Marie-Ève Tremblay1,2 1Axe Neurosciences, Centre de Recherche du CHU de Québec-Université Laval, 2Département de Médecine Moléculaire, Faculté de Médecine, Université Laval, 3Integrated Program in Neuroscience, McGill University, 4Center for Brain Immunology and Glia (BIG), University of Virginia This protocol describes a step-by-step workflow for immunofluorescent costaining of IBA1 and TMEM119, in addition to analysis of microglial density, distribution, and morphology, as well as peripheral myeloid cell infiltration in mouse brain tissue.
