Instituto Politecnico Nacional 5 articles published in JoVE Medicine Rapid Model to Evaluate the Anti-Obesity Potential of a Combination of Syzygium aromaticum (Clove) and Cuminun cyminum (Cumin) on C57BL6/j Mice Fed High-Fat Diet Rosa Martha Pérez Gutiérrez1, María Woolrich Arrioja1 1Laboratorio de Investigación de Productos Naturales. Escuela Superior de Ingeniería Química e Industrias Extractivas, Instituto Politécnico Nacional (IPN) Unidad Profesional Adolfo López Mateos S/N Av. Instituto Politécnico Nacional Ciudad de Mexico This work presents a protocol to explore the anti-obesity effect of two plants used together, for a 5-week duration. There was a combined administration of the extract and high-fat-diet (HFD) in the obese mice. The method can promote the benefits of plants in the treatment of obesity. Biochemistry Lipid Index Determination by Liquid Fluorescence Recovery in the Fungal Pathogen Ustilago Maydis Lucero Romero-Aguilar*1,2, Mónica Montero-Lomeli*3, Juan Pablo Pardo2, Guadalupe Guerra- Sánchez1 1Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, 2Facultad de Medicina, Universidad Nacional Autónoma de México, 3Instituto de Bioquímica Médica Leopoldo de Meis, Universidade Federal do Rio de Janeiro Here, we describe a protocol to obtain the lipid droplet index (LD index) to study the dynamics of triacylglycerols in cells cultured in high-throughput experiments. The LD index assay is an easy and reliable method that uses BODIPY 493/503. This assay does not need dispendious lipid extraction or microscopy analysis. Medicine Determination of the Relative Potency of an Anti-TNF Monoclonal Antibody (mAb) by Neutralizing TNF Using an In Vitro Bioanalytical Method Lilia Tierrablanca-Sánchez1, Víctor Pérez Medina Martínez1, Nancy D. Ramírez Ibañez1, Néstor O. Pérez Ramírez1, Francisco Luis Flores Ortiz1, Emilio Medina-Rivero2 1Research and Development, Probiomed, 2Bioprocess Research and Development Unit, Instituto Politecnico Nacional A protocol for the determination of the relative anti-apoptotic activity of an anti-TNFα mAb using a neutralization mechanism with WEHI 164 cells is presented here. This protocol is useful for comparing the neutralization strength of different molecules with the same biological functionality. Chemistry Determining the Chemical Composition of Corrosion Inhibitor/Metal Interfaces with XPS: Minimizing Post Immersion Oxidation Monika S. Walczak1, Perla Morales-Gil1,2, Turia Belashehr1, Kiran Kousar1, Paulina Arellanes Lozada1,3, Robert Lindsay1 1Corrosion and Protection Centre, School of Materials, The University of Manchester, 2Laboratorio de Caracterización de Materiales Sintéticos y Naturales, Instituto Mexicano del Petróleo, 3Departamento de Metalurgia y Materiales, Instituto Politécnico Nacional A protocol to avoid the oxidation of metallic substrates during sample transfer from an inhibited acidic solution to an X-ray photoelectron spectrometer is presented. Environment Development of Sulfidogenic Sludge from Marine Sediments and Trichloroethylene Reduction in an Upflow Anaerobic Sludge Blanket Reactor Claudia Guerrero-Barajas1, Alberto Ordaz1, Selene Montserrat García-Solares1, Claudio Garibay-Orijel1, Fernando Bastida-González2, Paola Berenice Zárate-Segura1 1Bioprocesses Department, Laboratory of Environmental Biotechnology, Unidad Profesional Interdisciplinaria de Biotecnología, Instituto Politécnico Nacional, 2Laboratory of Molecular Biology, Escuela Superior de Medicina, Instituto Politécnico Nacional Microbial sulfate reduction is a process of great importance in environmental biotechnology. The success of the sulfidogenic reactors depends among other factors on the microbial composition of the sludge. Here, we present a protocol to develop sulfidogenic sludge from hydrothermal vents sediments in a UASB reactor for reductive dechlorination purposes.
