Universidad Iberoamericana 3 articles published in JoVE Biology Immunohistochemistry Techniques to Analyze Cellular Proliferation and Neurogenesis in Rats Using the Thymidine Analog BrdU Mario Buenrostro-Jauregui*1, Alejandro Tapia-de-Jesús1, Jesus Mata1, Luis Miguel Rodríguez-Serrano1, María Elena Chávez-Hernández1, Florencia Mata1, Marco Monroy-Plasencia1,2, Aixchell Camila Alonso-Flores1, Perla Leal-Galicia*1 1Laboratorio de Neurociencias, Departamento de Psicología, Universidad Iberoamericana Ciudad de México, 2Facultad de Ciencias, Universidad Autónoma del Estado de México This paper presents four of the most common techniques for visualizing BrdU positive cells to measure adult neurogenesis in rats. This work includes instructions for reagent preparation, thymidine analog administration, transcardial perfusion, tissue preparation, peroxidase immunohistochemical reaction, immunofluorescence, signal amplification, counterstaining, microscopy imaging, and cell analysis. Behavior Simultaneous Monitoring of Wireless Electrophysiology and Memory Behavioral Test as a Tool to Study Hippocampal Neurogenesis Mario Buenrostro-Jáuregui*1, Luis Miguel Rodríguez-Serrano1, María Elena Chávez-Hernández1, Alejandro Tapia-de-Jesús1, Jesus Mata-Luevanos1, Florencia Mata1, Oscar Galicia-Castillo1, Daniel Tirado-Martínez1, Sylvia Ortega-Martinez2, Erik Bojorges-Valdez*3 1Laboratorio de Neurociencias, Departamento de Psicología, Universidad Iberoamericana Ciudad de México, 2Stoeling Co., 3Departamento de Estudios en Ingeniería para la Innovación, Universidad Iberoamericana Ciudad de México The protocol presented here provides information on the simultaneous electroencephalography (EEG) and behavioral assessment in real-time. We have discussed all steps involved in this protocol as an attractive solution for researchers in many fields of neuroscience, particularly in learning and memory areas. Behavior Three Laboratory Procedures for Assessing Different Manifestations of Impulsivity in Rats Rodrigo Sosa*1, Pablo Saavedra2, Roberto Niño de Rivera1, Gustavo Lago1, Patsy Moreno1, Oscar Galicia-Castillo1, César Hernández-Guerrero3, Mario Buenrostro-Jáuregui*1 1Laboratorio de Neurociencias, Universidad Iberoamericana, 2Departamento de Psicología, Universidad Anáhuac México Sur, 3Departamento de Salud, Universidad Iberoamericana We present three protocols that assess different forms of impulsivity in rats and other small mammals. Intertemporal choice procedures evaluate the tendency to discount the value of delayed outcomes. Differential reinforcement of low rates and feature-negative discrimination evaluate response inhibition capacity with and without punishment for inappropriate responses, respectively.
