Centro de Investigacion y de Estudios Avanzados del Instituto Politecnico Nacional 1 article published in JoVE Immunology and Infection Morphological and Compositional Analysis of Neutrophil Extracellular Traps Induced by Microbial and Chemical Stimuli Alexia Almaraz-Arreortua1, Sorely Adelina Sosa-Luis2, William de Jesús Ríos-Ríos1, María de los Ángeles Romero-Tlalolini3, Sergio Roberto Aguilar-Ruiz4, Rafael Baltiérrez-Hoyos3, Honorio Torres Aguilar1 1Clinical Immunology Research Department of Biochemical Sciences Faculty, Universidad Autónoma “Benito Juárez” de Oaxaca, 2Department of Molecular Biomedicine, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional, 3CONACYT- Medicine and Surgery Faculty, Universidad Autónoma “Benito Juárez” de Oaxaca, 4Molecular Immunology Research Department of Medicine and Surgery Faculty, Universidad Autónoma “Benito Juárez” de Oaxaca Presented here is a protocol for the induction and analysis of in vitro neutrophil extracellular traps (NETs). Quantification of DNA, cathelicidin (LL37), and enzyme activity yielded data that show the variability in the composition and morphology of NETs induced by microbial and chemical stimuli under similar controlled conditions.
