University of Colorado School of Medicine, University of Colorado-Denver 2 articles published in JoVE Neuroscience Measuring Transcellular Interactions through Protein Aggregation in a Heterologous Cell System Susana Restrepo1, Samantha L. Schwartz1, Matthew J. Kennedy1, Jason Aoto1 1Department of Pharmacology, University of Colorado Denver School of Medicine Here, we present an optimized protocol to rapidly and semiquantitatively measure ligand-receptor interactions in trans in a heterologous cell system using fluorescence microscopy. Medicine Murine Kidney Transplant Technique Robert Plenter1,2, Swati Jain3, Chelsea M. Ruller4, Trevor L. Nydam4, Alkesh H. Jani3,5 1Colorado Center for Transplantation Care, Research and Education, University of Colorado, Denver, 2Division of Pulmonary Sciences and Critical Care Medicine, University of Colorado, Denver, 3Department of Medicine, Division of Renal Diseases and Hypertension, Medical Center and University of Colorado, Denver, 4Department of Surgery, Division of Transplant Surgery, University of Colorado School of Medicine, University of Colorado-Denver, 5Renal Section, Denver Veterans Affairs Medical Center The goal of this manuscript is to describe the steps required to perform a kidney transplant in a mouse, paying particular attention to the details of the arterial anastomosis.
