Bolder Biopath Inc 1 article published in JoVE Cancer Research Identification, Histological Characterization, and Dissection of Mouse Prostate Lobes for In Vitro 3D Spheroid Culture Models Disharee Nath1,2, Julie R. White3,4, Gennady Bratslavsky1, Leszek Kotula1,2 1Department of Urology, SUNY Upstate Medical University, 2Department of Biochemistry and Molecular Biology, SUNY Upstate Medical University, 3Laboratory of Comparative Pathology, Memorial Sloan-Kettering Cancer Center, 4Boulder BioPATH, Inc Genetically engineered mice are useful models for investigating prostate cancer mechanisms. Here we present a protocol to identify and dissect prostate lobes from a mouse urogenital system, differentiate them based on histology, and isolate and culture the primary prostate cells in vitro as spheroids for downstream analyses.
