Colorado Center for Reproductive Medicine 1 article published in JoVE Biology Single Cell Collection of Trophoblast Cells in Peri-implantation Stage Human Embryos Deirdre M. Logsdon1, Rebecca A. Kile1, William B. Schoolcraft1, Rebecca L. Krisher1, Ye Yuan1 1Colorado Center for Reproductive Medicine Here, we describe a method for warming vitrified human blastocysts, culturing them through the implantation period in vitro, digesting them into single cells and collecting early trophoblast cells for further investigation.
